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- Table of Contents
Vcam1 antibody (A01199) performs well in IHC, WB, ELISA (Cap) and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 12 Publications
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IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199).
VCAM1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-VCAM1 Antibody (A01199) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
VCAM1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-VCAM1 Antibody (A01199) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Anti-VCAM1 Antibody Picoband®
Vcam1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | IHC, WB, ELISA (Cap) |
Tested Applications
Vcam1 antibody was positively detected by WB in:
mouse spleen tissue, mouse lung tissue, rat spleen tissue, rat lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Vcam1 antibody was positively detected by IHC in:
human kidney tissue, mouse spleen tissue, rat spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
ELISA (Cap)
1-5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Mouse
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Human
Tested
Recommend
Product Information
Product Name
Anti-VCAM1 Antibody Picoband®
View all VCAM1 Antibodies
View all VCAM1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse VCAM1 recombinant protein (Position: F25-L270).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
110 kDa
Calculated Molecular Weight
81.3 kDa
Background of Vcam1
CD106 (cluster of differentiation 106) also known as vascular cell adhesion molecule 1 (VCAM-1), is a protein that in humans is encoded by the VCAM1 gene. In inflammatory conditions and in cardiac allografts undergoing rejection, VCAM1 is upregulated in endothelium of postcapillary venules. Arterial expression of VCAM1 is also found in experimental models of atherosclerosis in the rabbit. This gene is mapped to chromosome 1 by Southern analysis of somatic cell hybrids. VCAM-1 functions as a cell adhesion molecule. The VCAM-1 protein mediates the adhesion of lymphocytes, monocytes, eosinophils, andbasophils to vascularendothelium. It also functions in leukocyte-endothelial cell signal transduction, and it may play a role in the development ofatherosclerosis and rheumatoid arthritis. CAM741 works similar to cotransin in that it represses the biosynthesis of VCAM1 cells by blocking the process of cotranslational translocation, which is dependent on the signal peptide ofVCAM1. Among the lung metastasis signature genes identified, several, includingVCAM1, were functionally validated.
Cite This Product
Anti-VCAM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01199)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
84%
Moderate Homology
Monkey
76%
Moderate Homology
Rabbit
76%
Moderate Homology
Human
75%
Moderate Homology
Sheep
66%
Low homology
Cow
63%
Low homology
Zebrafish
30%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Vcam1 antibody
Immunohistochemistry (IHC) protocol for Vcam1 antibody
Publications
Application:
Species:
Hormonal Treatment During Ex Vivo Lung Perfusion Ameliorates Brain Death Induced Inflammation
Published: 2026-03-13
Journal: ARTIFICIAL ORGANS
Application: IHC
Impact Factor: 2.30
Species: Rat
PMID: 41826278
Endothelial Gasdermin D Induces Mitochondrial Damage and Activates the STING Pathway in Lipopolysaccharide-Accelerated Atherosclerosis
Published: 2025-09-29
Journal: ANTIOXIDANTS & REDOX SIGNALING
Application: WB
Impact Factor: 6.10
Species: Mouse,Human
PMID: 41017337
Perihematomal Neurovascular Protection: Blocking HSP90 Reduces Blood Infiltration Associated with Inflammatory Effects Following Intracerebral Hemorrhage in Rates
Published: 2024-09-04
Journal: Translational Stroke Research
Application: WB
Impact Factor: 3.80
Species: Mouse
PMID: 39230786
Dihydromyricetin ameliorates diabetic renal fibrosis via regulating SphK1 to suppress the activation of NF-κB pathway
Published: 2024-06-29
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 4.20
Species: Mouse
PMID: 38945289
Punicalagin protects against the development of pancreatic injury and insulitis in rats with induced T1DM by reducing inflammation and oxidative stress
Published: 2022-06-06
Journal: MOLECULAR AND CELLULAR BIOCHEMISTRY
Application: ELISA
Impact Factor: 3.84
Species: Rat
PMID: 35666430
Sophocarpine Alleviates Injury-Induced Intima Hyperplasia of Carotid Arteries by Suppressing Inflammation in a Rat Model
Published: 2021-11-22
Journal: Journal of Clinical Medicine
Application: WB
Impact Factor: 4.24
Species: Rat
PMID: 34830730
Effects of Monocyte Chemotactic Protein-1 and Nuclear Factor of Kappa B Pathway in Rejection of Cardiac Allograft in Rat
Published: 2015-08-19
Journal: TRANSPLANTATION PROCEEDINGS
Application: IHC
Impact Factor: 0.98
Species: Rat
PMID: 26293090
Retinoic Acid Promotes Interleukin-4 Plasmid-Dimethylsulfoxide Topical Transdermal Delivery for Treatment of Psoriasis
Published: 2015-03-24
Journal: Annals of Dermatology
Application: IHC
Impact Factor: 1.39
Species: Mouse
PMID: 25834349
2,3,4′,5-Tetrahydroxystilbene-2-O-β-D-glucoside Suppresses Expression of Adhesion Molecules in Aortic Wall of Dietary Atherosclerotic Rats and Promonocytic U937 Cells
Published: 2013-04-12
Journal: CELL BIOCHEMISTRY AND BIOPHYSICS
Application: WB,IHC
Impact Factor: 1.91
Species: Rat
PMID: 23575939
The Interaction of Leukocytes and Adhesion Molecules in Mesenteric Microvessel Endothelial Cells after Internal Capsule Hemorrhage
Published: 2012-04-17
Journal: MICROCIRCULATION
Application: IHC
Impact Factor: 2.57
Species: Rat
PMID: 22510105
Protective Effects of Grape Seed Proanthocyanidin Extracts on Cerebral Cortex of Streptozotocin-Induced Diabetic Rats through Modulating AGEs/RAGE/NF-κB Pathway
Published: 2010-05-20
Journal: JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY
Application: IHC
Impact Factor: 0.93
Species: Rat
PMID: 20495289
Gene therapy for psoriasis in the K14-VEGF transgenic mouse model by topical transdermal delivery of interleukin-4 using ultradeformable cationic liposome
Published: 2010-05-14
Journal: JOURNAL OF GENE MEDICINE
Application: IHC
Impact Factor: 2.97
Species: Mouse
PMID: 20527041
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Product Promise
We promise Anti-VCAM1 Antibody Picoband® performs excellently in relevant applications for IHC, WB, ELISA (Cap). If it doesn't perform as promised, we will refund the product. Learn more
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A01199
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026