Mouse MMP-2 ELISA Kit PicoKine™

Mouse MMP-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Mmp2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. Cited in 19 publication(s).

Product Info Summary

SKU: EK0460
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

Product Name

Mouse MMP-2 ELISA Kit PicoKine™

See all MMP-2 products

SKU/Catalog Number

EK0460

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse MMP-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Mmp2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse MMP-2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0460)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A30-C662

Sensitivity

<10 pg/ml

Assay Range

625 pg/ml - 40,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0460 is reactive to Mmp2 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK0460 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0460-CAP Anti-Mouse Mmp2 Pre-coated 96-well strip microplate 1
EK0460-ST Mouse Mmp2 Standard 2 vials, 40 ng/tube
EK0460-DA Mouse Mmp2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)0625125025005000100002000040000
O.D0.0390.0940.1330.2200.4060.7261.2511.799

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect MMP-2, Dilution ratio of 1:50, concentration in serum is around 246ng/ml.

Some articles we found to cite concentrations of MMP-2 in samples: 18043506 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse MMP-2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)13473055163791452320116183
Standard deviation60.61140.53720.6781.31150.44744.41
CV (%)4.5%4.6%4.4%5.6%4.7%4.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of MMP-2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11347141413171303134542.753.1%
Sample 230553421323731183207139.394.3%
Sample 31637915222154701495315506536.163.4%
*number of samples for each test n=16.

Gene/Protein Information For Mmp2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P33434

Gene ID

17390

Gene Name

Mmp2

Full Name

72 kDa type IV collagenase

Weight

74.102kDa

Superfamily

peptidase M10A family

Alternative Names

72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase) Mmp2|Clg, Clg4a, Ge, GelA, MMP-, MMP-2|matrix metallopeptidase 2|72 kDa type IV collagenase|72 kDa gelatinase|72kD gelatinase|72kD type IV collagenase|72kDa gelatinase|72kDa type IV collagenase|gelatinase A|matrix metalloproteinase 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Mmp2, check out the Mmp2 Infographic

Mmp2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Mmp2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0460 has been cited in 19 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Resveratrol Suppresses Matrix Metalloproteinase-2 Activation Induced by Lipopolysaccharide in Mouse Osteoblasts via Interactions with AMP-Activated Protein %u2026

Marsdenia tenacissima extract suppresses tumor growth and angiogenesis in A20 mouse lymphoma

MMP-9-Dependent Serum-Borne Bioactivity Caused by Multiwalled Carbon Nanotube Exposure Induces Vascular Dysfunction via the CD36 Scavenger Receptor

Acute exacerbations of COPD are associated with significant activation of matrix metalloproteinase 9 irrespectively of airway obstruction, emphysema and infection

Role of Sphingosine Kinase/S1P Axis in ECM Remodeling of Cardiac Cells Elicited by Relaxin

Chen Y, Zeng C, Zhan Y, Wang H, Jiang X, Li W. Oncogene. 2017 Aug 17;36(33):4692-4705. doi: 10.1038/onc.2017.100. Epub 2017 Apr 10. Aberrant low expression of p85α in stromal fibroblasts promotes breast cancer cell metastasis through exosome-media...

Xie J, Huo S, Li Y, Dai J, Xu H, Yin ZQ. Cell Transplant. 2017 Jun 9;26(6):967-982. doi: 10.3727/096368917X694994. Epub 2017 Feb 9. Olfactory Ensheathing Cells Inhibit Gliosis in Retinal Degeneration by Downregulation of the M

Qin Y, Liu Xj, Li L, Liu Xj, Li Y, Gao Rj, Shao Rg, Zhen Ys. Oncol Rep. 2014 Jul;32(1):121-30. Doi: 10.3892/Or.2014.3169. Epub 2014 May 7. Mmp-2/9-Oriented Combinations Enhance Antitumor Efficacy Of Egfr/Her2-Targeting Fusion Proteins And Gemcitab...

Simmers P, Gishto A, Vyavahare N, Kothapalli Cr. Tissue Eng Part A. 2015 Apr;21(7-8):1455-70. Doi: 10.1089/Ten.Tea.2014.0363. Epub 2015 Mar 9. Nitric Oxide Stimulates Matrix Synthesis And Deposition By Adult Human Aortic Smooth Muscle Cells Within...

Xu Xl, Ling Dy, Zhu Qy, Fan Wj, Zhang W. Eur J Pharmacol. 2013 Jan 5;698(1-3):370-8. Doi: 10.1016/J.Ejphar.2012.11.019. Epub 2012 Nov 21. The Effect Of 2,3,4',5-Tetrahydroxystilbene-2-0-??-D Glucoside On Neointima Formation In A Rat Artery Balloon...

Tang Xp, Tang Gd, Fang Cy, Liang Zh, Zhang Ly. World J Gastroenterol. 2013 Mar 14;19(10):1582-92. Doi: 10.3748/Wjg.V19.I10.1582. Effects Of Ginsenoside Rh2 On Growth And Migration Of Pancreatic Cancer Cells.

Yuan H, Yang P, Zhou D, Gao W, Qiu Z, Fang F, Ding S, Xiao W. Mol Biol Rep. 2014 Aug;41(8):5157-65. Doi: 10.1007/S11033-014-3382-4. Epub 2014 May 10. Knockdown Of Sphingosine Kinase 1 Inhibits The Migration And Invasion Of Human Rheumatoid Arthrit...

Mazani M, Fard As, Baghi An, Nemati A, Mogadam Ra. J Pak Med Assoc. 2014 Jul;64(7):785-90. Effect Of Pomegranate Juice Supplementation On Matrix Metalloproteinases 2 And 9 Following Exhaustive Exercise In Young Healthy Males.

Liu Q,, Zou R, Zhou R, Gong C, Wang Z, Cai T, Tan C, Fang J. J Cell Biochem. 2015 Jul;116(7):1213-21. Doi: 10.1002/Jcb.25073. Mir-155 Regulates Glioma Cells Invasion And Chemosensitivity By P38 Isforms In Vitro.

Pan B, Ren H, Ma Y, Liu D, Yu B, Ji L, Pan L, Li J, Yang L, Lv X, Shen X, Chen B, Zhang Y, Willard B, He Y, Zheng L. Int J Cancer. 2012 Jul 1;131(1):70-82. Doi: 10.1002/Ijc.26341. Epub 2011 Aug 30. High-Density Lipoprotein Of Patients With Type 2 ...

Yu X, Zhu J, Mi M, Chen W, Pan Q, Wei M. Med Oncol. 2012 Mar;29(1):349-57. Doi: 10.1007/S12032-010-9770-2. Epub 2010 Dec 4. Anti-Angiogenic Genistein Inhibits Vegf-Induced Endothelial Cell Activation By Decreasing Ptk Activity And Mapk Activation.

Gishto A, Farrell K, Kothapalli Cr. J Biomed Mater Res A. 2015 Feb;103(2):693-708. Doi: 10.1002/Jbm.A.35217. Epub 2014 May 19. Tuning Composition And Architecture Of Biomimetic Scaffolds For Enhanced Matrix Synthesis By Murine Cardiomyocytes.

Kim Ks, Lee Ya, Choi Hm, Yoo Mc, Yang Hi. Rheumatol Int. 2012 Oct;32(10):3069-75. Epub 2011 Sep 13. Implication Of Mmp-9 And Urokinase Plasminogen Activator (Upa) In The Activation Of Pro-Matrix Metalloproteinase (Mmp)-13.

Li B, Xu Yj, Chu Xm, Gao Mh, Wang Xh, Nie Sm, Yang F, Lv Cy. Immunol Lett. 2013 Nov-Dec;156(1-2):68-81. Doi: 10.1016/J.Imlet.2013.09.025. Epub 2013 Sep 29. Molecular Mechanism Of Inhibitory Effects Of Cd59 Gene On Atherosclerosis In Apoe (-/-) Mice.

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8 Customer Q&As for Mouse MMP-2 ELISA Kit PicoKine™

Question

Q: how do I analyze ELISA data? I have obtained MMP-2 level in serum.

B. Puri

Verified customer

Asked: 2020-08-04

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-08-04

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Z. Liu

Verified customer

Asked: 2019-12-15

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-12-15

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-08-12

Question

Q: Can MMP-2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

M. Baker

Verified customer

Asked: 2019-03-08

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-03-08

Question

Q: is it okay to use citrate plasma as samples in Mouse MMP-2 Picokine® ELISA Kit (Catalog # EK0460)?

D. Huang

Verified customer

Asked: 2019-03-08

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of MMP-2 causing disruption of its protein structure. MMP-2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the MMP-2 ELISA, treating samples with a number of anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-03-08

Question

Q: can you suggest the dilution ratio of serum samples for detection of MMP-2 in Mouse serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

D. Dhar

Verified customer

Asked: 2018-07-06

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the MMP-2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-07-06

Question

Q: if the enzyme conjugated MMP-2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the MMP-2 antigen?

Verified Customer

Verified customer

Asked: 2017-01-31

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-01-31

Question

Q: which procedure should I follow in order to thaw whole blood sample for MMP-2 ELISA after freezing?

W. Wilson

Verified customer

Asked: 2014-07-05

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test MMP-2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2014-07-05

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