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- Table of Contents
VCAM1 antibody (A01199-1) performs well in ELISA, IHC and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199-1).
VCAM1 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VCAM1 Antibody (A01199-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
VCAM1 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VCAM1 Antibody (A01199-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-VCAM1 Antibody Picoband®
VCAM1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, IHC |
Tested Applications
VCAM1 antibody was positively detected by IHC in:
human appendix tissue, human tonsil tissue, human kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
ELISA (Cap)
1-5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
ELISA
Human
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-VCAM1 Antibody Picoband®
View all VCAM1 Antibodies
View all VCAM1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human VCAM1 recombinant protein (Position: F25-L270).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Calculated Molecular Weight
81.3 kDa
Background of VCAM1
CD106 (cluster of differentiation 106) also known as vascular cell adhesion molecule 1 (VCAM-1), is a protein that in humans is encoded by the VCAM1 gene. In inflammatory conditions and in cardiac allografts undergoing rejection, VCAM1 is upregulated in endothelium of postcapillary venules. Arterial expression of VCAM1 is also found in experimental models of atherosclerosis in the rabbit. This gene is mapped to chromosome 1 by Southern analysis of somatic cell hybrids. VCAM-1 functions as a cell adhesion molecule. The VCAM-1 protein mediates the adhesion of lymphocytes, monocytes, eosinophils, andbasophils to vascularendothelium. It also functions in leukocyte-endothelial cell signal transduction, and it may play a role in the development ofatherosclerosis and rheumatoid arthritis. CAM741 works similar to cotransin in that it represses the biosynthesis of VCAM1 cells by blocking the process of cotranslational translocation, which is dependent on the signal peptide ofVCAM1. Among the lung metastasis signature genes identified, several, includingVCAM1, were functionally validated.
Cite This Product
Anti-VCAM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01199-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
95%
High Homology
Rabbit
87%
Moderate Homology
Rat
75%
Moderate Homology
Dog
75%
Moderate Homology
Mouse
75%
Moderate Homology
Sheep
72%
Moderate Homology
Cow
71%
Moderate Homology
Zebrafish
28%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for VCAM1 antibody
Publications
Application:
Species:
Malvidin and its Glycosides from Vaccinium ashei Improve Endothelial Function by Anti-inflammatory and Angiotensin I-Converting Enzyme Inhibitory Effects
Published: 2018-01-01
Journal: Natural Product Communications
Application: WB
Impact Factor: 0.81
Species: Human
Quercetin, Hyperin, and Chlorogenic Acid Improve Endothelial Function by Antioxidant, Antiinflammatory, and ACE Inhibitory Effects
Published: 2017-04-13
Journal: JOURNAL OF FOOD SCIENCE
Application: WB
Impact Factor: 1.82
Species: Human
PMID: 28407238
Uric Acid Induces Endothelial Dysfunction by Activating the HMGB1/RAGE Signaling Pathway
Published: 2017-01-01
Journal: Biomed Research International
Application: WB
Impact Factor: 2.48
Species: Human
PMID: 28116308
A novel anti-inflammatory mechanism of high density lipoprotein through up-regulating annexin A1 in vascular endothelial cells
Published: 2016-03-21
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
Application: ELISA
Impact Factor: 4.78
Species: Mouse
PMID: 27012521
Anti-Inflammatory Effect of the Blueberry Anthocyanins Malvidin-3-Glucoside and Malvidin-3-Galactoside in Endothelial Cells
Published: 2014-08-21
Journal: MOLECULES
Application: WB
Impact Factor: 2.10
Species: Human
PMID: 25153881
Inhibitory effect of Malvidin on TNF-α-induced inflammatory response in endothelial cells
Published: 2013-12-11
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 2.59
Species: Human
PMID: 24333549
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Product Promise
We promise Anti-VCAM1 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A01199-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026