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Product Info Summary
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Anti-VCAM1 Antibody Picoband™
Boster Bio Anti-VCAM1 Antibody Picoband™ catalog # A01199-1. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VCAM1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01199-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived human VCAM1 recombinant protein (Position: F25-L270).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A01199-1 is reactive to VCAM1 in Human
A01199-1 is guaranteed for ELISA, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of VCAM1 using anti-VCAM1 antibody (A01199-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human VCAM1 protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCAM1 antigen affinity purified polyclonal antibody (Catalog # A01199-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCAM1 at approximately 95KD. The expected band size for VCAM1 is at 74KD.
Gene/Protein Information For VCAM1 (Source: Uniprot.Org, NCBI)
Vascular cell adhesion protein 1
CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1 VCAM1 CD106, INCAM-100 vascular cell adhesion molecule 1 vascular cell adhesion protein 1|CD106 antigen*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on VCAM1, check out the VCAM1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for VCAM1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
A01199-1 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
LDL Receptor Gene-ablated Hamsters: A Rodent Model of Familial Hypercholesterolemia With Dominant Inheritance and Diet-induced Coronary Atherosclerosis
Mesenchymal stromal cells ameliorate oxidative stress-induced islet endothelium apoptosis and functional impairment via Wnt4-?-catenin signaling
Trimethylamine N-oxide in atherogenesis: impairing endothelial self-repair capacity and enhancing monocyte adhesion
Retinoic Acid Promotes Interleukin-4 Plasmid-Dimethylsulfoxide Topical Transdermal Delivery for Treatment of Psoriasis
TSP-1 Secreted by Bone Marrow Stromal Cells Contributes to Retinal Ganglion Cell Neurite Outgrowth and Survival
Isolation and morphological characterization of ovine amniotic fluid mesenchymal stem cells
Ma, G., Pan, B., Ren, S., Guo, C., Guo, Y., Wei, L.,…, & Chen, B. (2017). 15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell. Lipids in Health and Disease, 16(1), 137. doi: 10.1186/s12944-017-0518-2
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