Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | A01199-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-VCAM1 Antibody Picoband®
SKU/Catalog Number
A01199-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VCAM1 Antibody Picoband® catalog # A01199-1. Tested in ELISA, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VCAM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01199-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human VCAM1 recombinant protein (Position: F25-L270).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01199-1 is reactive to VCAM1 in Human
Calculated molecular weight
81.3 kDa
Background of VCAM1
CD106 (cluster of differentiation 106) also known as vascular cell adhesion molecule 1 (VCAM-1), is a protein that in humans is encoded by the VCAM1 gene. In inflammatory conditions and in cardiac allografts undergoing rejection, VCAM1 is upregulated in endothelium of postcapillary venules. Arterial expression of VCAM1 is also found in experimental models of atherosclerosis in the rabbit. This gene is mapped to chromosome 1 by Southern analysis of somatic cell hybrids. VCAM-1 functions as a cell adhesion molecule. The VCAM-1 protein mediates the adhesion of lymphocytes, monocytes, eosinophils, andbasophils to vascularendothelium. It also functions in leukocyte-endothelial cell signal transduction, and it may play a role in the development ofatherosclerosis and rheumatoid arthritis. CAM741 works similar to cotransin in that it represses the biosynthesis of VCAM1 cells by blocking the process of cotranslational translocation, which is dependent on the signal peptide ofVCAM1. Among the lung metastasis signature genes identified, several, includingVCAM1, were functionally validated.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01199-1 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
ELISA (Cap), 1-5μg/ml, -
Positive Control
IHC: human appendix tissue, human tonsil tissue, human kidney tissue
Validation Images & Assay Conditions
Click image to see more details
IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199-1).
VCAM1 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VCAM1 Antibody (A01199-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199-1).
VCAM1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VCAM1 Antibody (A01199-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Sandwich ELISA - Recombinant human VCAM1 protein standard curve.
Use in combination with reagents from Human VCAM1 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0537).
Click image to see more details
IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199-1).
VCAM1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VCAM1 Antibody (A01199-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199-1).
VCAM1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 9.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-VCAM1 Antibody (A01199-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-VCAM1 Antibody Picoband® (A01199-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-VCAM1 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-VCAM1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
