Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine™

Rat IL-18 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. Cited in 21 publication(s).

Product Info Summary

SKU: EK0592
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine™

See all IL-18/IL-1F4 products

SKU/Catalog Number

EK0592

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat IL-18 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0592)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: H37-S194

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0592 is reactive to Il18 in Rat samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0592 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0592-CAP Anti-Rat Il18 Pre-coated 96-well strip microplate 1
EK0592-ST Rat Il18 Standard 2 vials, 10 ng/tube
EK0592-DA Rat Il18 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0270.0690.1010.1730.3230.5771.0921.856

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-18/IL-1F4, Dilution ratio of 1:1, concentration in serum is 4-30pg/ml.

Some articles we found to cite concentrations of IL-18/IL-1F4 in samples: 23983360 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2916651129165490
Standard deviation1.972.222.992.29.0729.4
CV (%)6.8%4.7%4.5%7.6%5.5%6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-18/IL-1F4 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 129293028290.72.4%
Sample 21661491651521587.584.7%
Sample 351148952049950411.752.3%
*number of samples for each test n=16.

Gene/Protein Information For Il18 (Source: Uniprot.Org, NCBI)

Uniprot ID

P97636

Gene ID

29197

Gene Name

Il18

Full Name

Interleukin-18

Weight

22.303kDa

Superfamily

IL-1 family

Alternative Names

Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18 Il18|IL-1 gamma, IL-18|interleukin 18|interleukin-18|IFN-gamma-inducing factor|interferon gamma-inducing factor|interleukin-1 gamma

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il18, check out the Il18 Infographic

Il18 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il18: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0592 has been cited in 21 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Huang Y,Sun X,Juan Z,Zhang R,Wang R,Meng S,Zhou J,Li Y,Xu K,Xie K.Dexmedetomidine attenuates myocardial ischemia-reperfusion injury in vitro by inhibiting NLRP3 Inflammasome activation.BMC Anesthesiol.2021 Apr 6;21(1):104.doi:10.1186/s12871-021-01334-5.PMID:33823789.
Species: Rat
EK0592 usage in article: APP:ELISA, SAMPLE:CMS AND CFS, DILUTION:NA

Abd El-Hameed,A.M.Polydatin-loaded chitosan nanoparticles ameliorates early diabetic nephropathy by attenuating oxidative stress and inflammatory responses in streptozotocin-induced diabetic rat. J Diabetes Metab Disord (2020).https://doi.org/10.1007/s402
Species: Rat
EK0592 usage in article: APP:ELISA, SAMPLE:BLOOD, DILUTION:NA

Yudi Zhang,Chunhe Tao,Donglin Du, et al.Astragaloside IV Protects Against Diabetic Nephropathy by Inhibiting Pyroptosis Based on NOX4/TXNIP/NLRP3 Pathway. Authorea. December 01, 2020. DOI: 10.22541/au.160684072.22355285/v1
Species: Rat
EK0592 usage in article: APP:ELISA, SAMPLE:SERUM AND KIDNEY, DILUTION:NA

Wan SY,Li GS,Tu C,Chen WL,Wang XW,Wang YN,Peng LB,Tan F.MicroNAR-194-5p hinders the activation of NLRP3 inflammasomes and alleviates neuroinflammation during intracerebral hemorrhage by blocking the interaction between TRAF6 and NLRP3.Brain Res.2020 Dec 2
Species: Rat
EK0592 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA

Fan X,Ma W,Zhang Y,Zhang L.P2X7 Receptor (P2X7R) of Microglia Mediates Neuroinflammation by Regulating (NOD)-Like Receptor Protein 3 (NLRP3) Inflammasome-Dependent Inflammation After Spinal Cord Injury. Med Sci Monit.2020 Sep 21;26:e925491.doi:10.12659/MS
Species: Rat
EK0592 usage in article: APP:ELISA, SAMPLE:SPINAL CORD, DILUTION:NA

Liu Z,Meng Y,Miao Y,Yu L,Yu Q. Propofol reduces renal ischemia/reperfusion-induced acute lung injury by stimulating sirtuin 1 and inhibiting pyroptosis. Aging(Albany NY).2020 Dec1;12.doi:10.18632/aging.202191.Epub ahead of print.PMID:33260147.
Species: Rat
EK0592 usage in article: APP:ELISA, SAMPLE:NR8383 CELL SUPERNATANTS AND RAT LUNG TISSUE, DILUTION:NA

Anti-inflammatory and Antioxidative Activities of Safranal in the Reduction of Renal Dysfunction and Damage that Occur in Diabetic Nephropathy

Curcumin immune-mediated and anti-apoptotic mechanisms protect against renal ischemia/reperfusion and distant organ induced injuries

Brilliant Blue G Inhibits Inflammasome Activation and Reduces Disruption of Blood-Spinal Cord Barrier Induced by Spinal Cord Injury in Rats

Nrf2 inhibits NLRP3 inflammasome activation through regulating Trx1/TXNIP complex in cerebral ischemia reperfusion injury

Neuroprotective Effects of Luteolin Against Spinal Cord Ischemia-Reperfusion Injury by Attenuation of Oxidative Stress, Inflammation, and Apoptosis

The effects of IL-18BP on mRNA expression of inflammatory cytokines and apoptotic genes in renal injury induced by infrarenal aortic occlusion

Anti-inflammatory and antiapoptotic effect of interleukine-18 binding protein on the spinal cord ischemia-reperfusion injury

Interleukin 18--binding protein ameliorates liver ischemia--reperfusion injury

Theaflavin alleviates inflammatory response and brain injury induced by cerebral hemorrhage via inhibiting the nuclear transcription factor kappa ?-related pathway in rats

MiR-30b Attenuates Neuropathic Pain by Regulating Voltage-Gated Sodium Channel Nav1.3 in Rats

Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats

Effect of interferon-? on NF-?B and cytokine IL-18 and IL-27 in acute pancreatitis

Li J, Hu SB, Wang LY, Zhang X, Zhou X, Yang B, Li JH, Xiong J, Liu N, Li Y, Wu YZ, Zheng QC. Oncogene. 2017 Nov 30;36(48):6725-6737. doi: 10.1038/onc.2017.272. Epub 2017 Aug 7. Autophagy-dependent generation of Axin2+ cancer stem-like cells promot...

Tian, R., Zhu, Y., Yao, J., Meng, X., Wang, J., Xie, H., & Wang, R. (2017). NLRP3 participates in the regulation of EMT in bleomycin-induced pulmonary fibrosis. Experimental Cell Research. Advance online publication. doi: 10.1016/j.yexcr.2017.05.028

Yang Yj, Shen Y, Chen Sh, Ge Xr. World J Gastroenterol. 2005 Aug 7;11(29):4524-9. Role Of Interleukin 18 In Acute Lung Inflammation Induced By Gut Ischemia Reperfusion.

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10 Customer Q&As for Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine™

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

W. Wilson

Verified customer

Asked: 2020-11-17

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-11-17

Question

Q: is it okay to use citrate plasma as samples in Rat IL-18 Picokine® ELISA Kit (Catalog # EK0592)?

Verified Customer

Verified customer

Asked: 2020-09-22

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of IL-18 causing disruption of its protein structure. IL-18 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IL-18 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-09-22

Question

Has the serum / plasma MRD (Minimal Required Dilution) been calculated for EK0592 kit?

Verified customer

Asked: 2020-04-05

Answer

The serum / plasma MRD (Minimal Required Dilution) for Rat IL-18 ELISA Kit PicoKine EK0592 is 1:1.

Boster Scientific Support

Answered: 2020-04-07

Question

Q: can you suggest the dilution ratio of serum samples for detection of IL-18 in Rat serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

V. Mitchell

Verified customer

Asked: 2019-08-24

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-18 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-08-24

Question

Was baculovirus expression system used for the manufacturing process of EK0592? How about recombinant Rat IL-18 standard and antibodies? Is this a recombinant protein?

Verified customer

Asked: 2019-05-15

Answer

For the Rat IL-18 ELISA Kit PicoKine™ (EK0592), E.coli expression system is used and it is a recombinant protein.

Boster Scientific Support

Answered: 2019-05-16

Question

Has EK0592 been validated on urine samples?

Verified customer

Asked: 2019-05-15

Answer

We have not tested nor validated the Rat IL-18 ELISA Kit PicoKine™ (EK0592) with urine sample. In theory the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. For sample types that we have not tested for, we suggest to run pilot experiments and decide the optimal sample dilution.

Boster Scientific Support

Answered: 2019-05-16

Question

Q: Can IL-18 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2017-06-15

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2017-06-15

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

H. Williams

Verified customer

Asked: 2017-04-16

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2017-04-16

Question

Q: if the enzyme conjugated IL-18 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-18 antigen?

Verified Customer

Verified customer

Asked: 2016-03-19

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-03-19

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification IL-18 using the EK0592 Rat IL-18 Picokine® ELISA Kit?

D. Davis

Verified customer

Asked: 2014-01-28

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2014-01-28

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