Rat IL-18 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IL-18. 96wells/kit, with removable strips. Cited in 7 publication(s).
Rat IL-18 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Rat IL-18 ELISA Kit PicoKine™
See all Il18 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Rat IL18 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL18 tissue specificity:|
|Cite This Product||Rat IL-18 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0592)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen||Expression system for standard: E.coli; Immunogen sequence: H37-S194|
|Antibody Clonalities||Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Protein Target Info (Source: Uniprot.org)
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.|
|Background||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone.|
Kit Components And Assay QC Data Details Of Rat IL-18 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0592-CAP||96-well plate precoated with anti-Rat Il18 antibody||1|
|EK0592-ST||lyophilized recombinant Rat Il18 standard||10ng/tube|
|EK0592-DA||biotinylated anti-Rat Il18 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0592 Rat IL-18 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Rat IL-18 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Rat IL-18 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 7
|Yang Yj, Shen Y, Chen Sh, Ge Xr. World J Gastroenterol. 2005 Aug 7;11(29):4524-9. Role Of Interleukin 18 In Acute Lung Inflammation Induced By Gut Ischemia Reperfusion.||PubMed ID 16052682|
|Tian, R., Zhu, Y., Yao, J., Meng, X., Wang, J., Xie, H., & Wang, R. (2017). NLRP3 participates in the regulation of EMT in bleomycin-induced pulmonary fibrosis. Experimental Cell Research. Advance online publication. doi: 10.1016/j.yexcr.2017.05.028||PubMed ID 28591554|
|Li J, Hu SB, Wang LY, Zhang X, Zhou X, Yang B, Li JH, Xiong J, Liu N, Li Y, Wu YZ, Zheng QC. Oncogene. 2017 Nov 30;36(48):6725-6737. doi: 10.1038/onc.2017.272. Epub 2017 Aug 7. Autophagy-dependent generation of Axin2+ cancer stem-like cells promot...||PubMed ID 28783177|
|Effect of interferon-? on NF-?B and cytokine IL-18 and IL-27 in acute pancreatitis||PubMed ID 23725508|
|Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats||PubMed ID 27524893|
|MiR-30b Attenuates Neuropathic Pain by Regulating Voltage-Gated Sodium Channel Nav1.3 in Rats||PubMed ID 28588637|
|Theaflavin alleviates inflammatory response and brain injury induced by cerebral hemorrhage via inhibiting the nuclear transcription factor kappa ?-related pathway in rats||PubMed ID 29928110|
Order Product (EK0592)
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.See Details
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.