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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat IL-18 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0592)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: H37-S194
15.6 pg/ml - 1,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0592 is reactive to Il18 in Rat samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0592 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0592-CAP||Anti-Rat Il18 Pre-coated 96-well strip microplate||1|
|EK0592-ST||Rat Il18 Standard||2 vials, 10 ng/tube|
|EK0592-DA||Rat Il18 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
Click image to see more details
Rat IL-18 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-18/IL-1F4, Dilution ratio of 1:1, concentration in serum is 4-30pg/ml.
Some articles we found to cite concentrations of IL-18/IL-1F4 in samples: 23983360 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of IL-18/IL-1F4 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Il18 (Source: Uniprot.Org, NCBI)
Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18 Il18|IL-1 gamma, IL-18|interleukin 18|interleukin-18|IFN-gamma-inducing factor|interferon gamma-inducing factor|interleukin-1 gamma*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Il18, check out the Il18 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il18: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0592 has been cited in 21 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Huang Y,Sun X,Juan Z,Zhang R,Wang R,Meng S,Zhou J,Li Y,Xu K,Xie K.Dexmedetomidine attenuates myocardial ischemia-reperfusion injury in vitro by inhibiting NLRP3 Inflammasome activation.BMC Anesthesiol.2021 Apr 6;21(1):104.doi:10.1186/s12871-021-01334-5.PMID:33823789.
EK0592 usage in article: APP:ELISA, SAMPLE:CMS AND CFS, DILUTION:NA
Abd El-Hameed,A.M.Polydatin-loaded chitosan nanoparticles ameliorates early diabetic nephropathy by attenuating oxidative stress and inflammatory responses in streptozotocin-induced diabetic rat. J Diabetes Metab Disord (2020).https://doi.org/10.1007/s402
EK0592 usage in article: APP:ELISA, SAMPLE:BLOOD, DILUTION:NA
Yudi Zhang,Chunhe Tao,Donglin Du, et al.Astragaloside IV Protects Against Diabetic Nephropathy by Inhibiting Pyroptosis Based on NOX4/TXNIP/NLRP3 Pathway. Authorea. December 01, 2020. DOI: 10.22541/au.160684072.22355285/v1
EK0592 usage in article: APP:ELISA, SAMPLE:SERUM AND KIDNEY, DILUTION:NA
Wan SY,Li GS,Tu C,Chen WL,Wang XW,Wang YN,Peng LB,Tan F.MicroNAR-194-5p hinders the activation of NLRP3 inflammasomes and alleviates neuroinflammation during intracerebral hemorrhage by blocking the interaction between TRAF6 and NLRP3.Brain Res.2020 Dec 2
EK0592 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA
Fan X,Ma W,Zhang Y,Zhang L.P2X7 Receptor (P2X7R) of Microglia Mediates Neuroinflammation by Regulating (NOD)-Like Receptor Protein 3 (NLRP3) Inflammasome-Dependent Inflammation After Spinal Cord Injury. Med Sci Monit.2020 Sep 21;26:e925491.doi:10.12659/MS
EK0592 usage in article: APP:ELISA, SAMPLE:SPINAL CORD, DILUTION:NA
Liu Z,Meng Y,Miao Y,Yu L,Yu Q. Propofol reduces renal ischemia/reperfusion-induced acute lung injury by stimulating sirtuin 1 and inhibiting pyroptosis. Aging(Albany NY).2020 Dec1;12.doi:10.18632/aging.202191.Epub ahead of print.PMID:33260147.
EK0592 usage in article: APP:ELISA, SAMPLE:NR8383 CELL SUPERNATANTS AND RAT LUNG TISSUE, DILUTION:NA
Anti-inflammatory and Antioxidative Activities of Safranal in the Reduction of Renal Dysfunction and Damage that Occur in Diabetic Nephropathy
Curcumin immune-mediated and anti-apoptotic mechanisms protect against renal ischemia/reperfusion and distant organ induced injuries
Brilliant Blue G Inhibits Inflammasome Activation and Reduces Disruption of Blood-Spinal Cord Barrier Induced by Spinal Cord Injury in Rats
Nrf2 inhibits NLRP3 inflammasome activation through regulating Trx1/TXNIP complex in cerebral ischemia reperfusion injury
Neuroprotective Effects of Luteolin Against Spinal Cord Ischemia-Reperfusion Injury by Attenuation of Oxidative Stress, Inflammation, and Apoptosis
The effects of IL-18BP on mRNA expression of inflammatory cytokines and apoptotic genes in renal injury induced by infrarenal aortic occlusion
Anti-inflammatory and antiapoptotic effect of interleukine-18 binding protein on the spinal cord ischemia-reperfusion injury
Interleukin 18--binding protein ameliorates liver ischemia--reperfusion injury
Theaflavin alleviates inflammatory response and brain injury induced by cerebral hemorrhage via inhibiting the nuclear transcription factor kappa ?-related pathway in rats
MiR-30b Attenuates Neuropathic Pain by Regulating Voltage-Gated Sodium Channel Nav1.3 in Rats
Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats
Effect of interferon-? on NF-?B and cytokine IL-18 and IL-27 in acute pancreatitis
Li J, Hu SB, Wang LY, Zhang X, Zhou X, Yang B, Li JH, Xiong J, Liu N, Li Y, Wu YZ, Zheng QC. Oncogene. 2017 Nov 30;36(48):6725-6737. doi: 10.1038/onc.2017.272. Epub 2017 Aug 7. Autophagy-dependent generation of Axin2+ cancer stem-like cells promot...
Tian, R., Zhu, Y., Yao, J., Meng, X., Wang, J., Xie, H., & Wang, R. (2017). NLRP3 participates in the regulation of EMT in bleomycin-induced pulmonary fibrosis. Experimental Cell Research. Advance online publication. doi: 10.1016/j.yexcr.2017.05.028
Yang Yj, Shen Y, Chen Sh, Ge Xr. World J Gastroenterol. 2005 Aug 7;11(29):4524-9. Role Of Interleukin 18 In Acute Lung Inflammation Induced By Gut Ischemia Reperfusion.
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