Anti-Neurofilament NF-M Antibody

Product Name

Anti-Neurofilament NF-M Antibody

View all NF-M Antibodies

SKU/Catalog Number

M06821-3

Size

100ul

Description

Boster Bio Anti-Neurofilament NF-M Antibody catalog # M06821-3. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Chicken, Equine, Pig.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Neurofilament NF-M Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06821-3)

Host

Chicken

Contents

Purified IgY preparation at 20-30 mg/mL with 5mM NaN₃

Clonality

Polyclonal

Immunogen

Recombinant construct containing the C-terminus of the human sequence (amino acids 708-877) expressed in and purified from E. coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M06821-3 is reactive to NEFM in Bovine, Chicken, Equine, Pig

Applications

M06821-3 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

102.472kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Use 1:10,000 for WB, 1:1,000 for ICC

Validation Images & Assay Conditions

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Western blot analysis of different neuronal tissue and cell lysates using chicken pAb to NF-M, M06821-3, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH/3T3 cells, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. Strong band at 145kDa corresponds to rodent NF-M, and about 160kDa band corresponds to human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties. NF-M is not expressed in HeLa and other cell lines tested.

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Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to NF-M, M06821-3, dilution 1:1,000 in red, and costained with mouse mAb to CNP, dilution 1:500 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45M, and free-floating sections were stained with the above antibodies. The NF-M antibody labels the network of axons of basket neurons and other neurons. The CNP antibody stains oligodendrocytes, cells that create myelin sheaths around axons.

Gene/Protein Information For NEFM (Source: Uniprot.org, NCBI)

Alternative Names

NEF3; NEF3neurofilament medium polypeptide; NEFM; Neurofilament 3; neurofilament, medium polypeptide 150kDa; neurofilament, medium polypeptide; neurofilament-3 (150 kD medium); NFM; NF-M; NF-M160 kDa neurofilament protein; NFMNeurofilament triplet M protein NEFM NEF3, NF-M, NFM neurofilament medium neurofilament medium polypeptide|160 kDa neurofilament protein|neurofilament 3|neurofilament triplet M protein|neurofilament, medium polypeptide 150kDa|neurofilament-3 (150 kD medium)

For more info on NEFM, check out the NEFM Infographic

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In this infographic, you will see the following information for NEFM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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