Click image to see more details
Product Info Summary
| SKU: | M00109-1 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal Antibody, Clone#RM320
SKU/Catalog Number
M00109-1
Size
100uL
Form
Liquid
Description
Boster Bio Anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal Antibody, Clone#RM320 (Catalog # M00109-1). Tested in IHC, WB, ICC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal Antibody, Clone#RM320 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00109-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM320
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human PD-L1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human PD-L1 (CD274) (B7-H1)
Reactive Species
M00109-1 is reactive to CD274 in Human
Applications
M00109-1 is guaranteed for IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
33.275kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:200-1:1000 dilution
WB: 1:500-1:1000 dilution
Immunocytochemistry (ICC): 1:200-1:500 dilution
Validation Images & Assay Conditions
Click image to see more details
Western Blot of lysates H1975, DU145, and LNCaP cells, using anti-PD-L1 rabbit monoclonal antibody (Clone RM320) at a 1:1000 dilution.
Click image to see more details
Immunohistochemical staining of formalin fixed and paraffin embedded human lung cancer tissue section using anti-PD-L1 rabbit monoclonal antibody (Clone RM320) at a 1:1000 dilution.
Click image to see more details
Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-PD-L1 rabbit monoclonal antibody (Clone RM320) at a 1:250 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CD274 (Source: Uniprot.org, NCBI)
Gene Name
CD274
Full Name
Programmed cell death 1 ligand 1
Weight
33.275kDa
Superfamily
immunoglobulin superfamily
Alternative Names
B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1 CD274 B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1 CD274 molecule programmed cell death 1 ligand 1|B7 homolog 1|CD274 antigen|PDCD1 ligand 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD274, check out the CD274 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD274: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal Antibody, Clone#RM320 (M00109-1)
Hello CJ!
No publications found for M00109-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal Antibody, Clone#RM320?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal Antibody, Clone#RM320
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
11 Customer Q&As for Anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal Antibody, Clone#RM320
Question
We bought anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
I viewed the website and datasheets of our anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2020-02-21
Question
Is this M00109-1 anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 reactive to the isotypes of CD274?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The immunogen of M00109-1 anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 is A peptide corresponding to the C-terminus of human PD-L1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-27
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for gastric carcinoma using anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 M00109-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-05
Question
Our lab want to know about using your anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 for toxin transport studies. Has this antibody been tested with western blotting on human tonsil tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
I appreciate your inquiry. This M00109-1 anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 is tested on lncap cells, human tonsil tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-30
Question
We have observed staining in human chorionic villus. Do you have any suggestions? Is anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 supposed to stain chorionic villus positively?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
Based on literature chorionic villus does express CD274. Based on Uniprot.org, CD274 is expressed in chorionic villus, placenta, gastric carcinoma, lung, liver, among other tissues. Regarding which tissues have CD274 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Placenta, Pubmed ID: 11015443, 14702039
Boster Scientific Support
Answered: 2019-07-08
Question
See below the WB image, lot number and protocol we used for gastric carcinoma using anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 M00109-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-05-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-30
Question
I am looking for to test anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 M00109-1 on human gastric carcinoma for research purposes, then I may be interested in using anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 M00109-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-03-30
Answer
The products we sell, including anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 M00109-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-30
Question
Would anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 M00109-1 work for IHC with gastric carcinoma?
Verified Customer
Verified customer
Asked: 2017-11-03
Answer
According to the expression profile of gastric carcinoma, CD274 is highly expressed in gastric carcinoma. So, it is likely that anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 M00109-1 will work for IHC with gastric carcinoma.
Boster Scientific Support
Answered: 2017-11-03
Question
My colleagues were well pleased with the WB result of your anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone
B. Walker
Verified customer
Asked: 2016-07-07
Answer
From literature, lung does express CD274. Generally CD274 expresses in cell membrane. Regarding which tissues have CD274 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Placenta, Pubmed ID: 11015443, 14702039
Boster Scientific Support
Answered: 2016-07-07
Question
I was wanting to use your anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 for IHC for human gastric carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human gastric carcinoma identification?
C. Thomas
Verified customer
Asked: 2015-08-31
Answer
As indicated on the product datasheet, M00109-1 anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human gastric carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-08-31
Question
We are currently using anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 M00109-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
R. Krishna
Verified customer
Asked: 2013-06-18
Answer
The anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 (M00109-1) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-18
