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Product Info Summary
| SKU: | M00109-1 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal Antibody, Clone#RM320
SKU/Catalog Number
M00109-1
Size
100uL
Form
Liquid
Description
Boster Bio Anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal Antibody, Clone#RM320 (Catalog # M00109-1). Tested in IHC, WB, ICC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal Antibody, Clone#RM320 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00109-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM320
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human PD-L1
Cross-reactivity
This antibody reacts to human PD-L1 (CD274) (B7-H1)
Reactive Species
M00109-1 is reactive to Cd274 in Human
Calculated molecular weight
32.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00109-1 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:200-1:1000 dilution
WB: 1:500-1:1000 dilution
Immunocytochemistry (ICC): 1:200-1:500 dilution
Validation Images & Assay Conditions
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Western Blot of lysates H1975, DU145, and LNCaP cells, using anti-PD-L1 rabbit monoclonal antibody (Clone RM320) at a 1:1000 dilution.
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Immunohistochemical staining of formalin fixed and paraffin embedded human lung cancer tissue section using anti-PD-L1 rabbit monoclonal antibody (Clone RM320) at a 1:1000 dilution.
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Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-PD-L1 rabbit monoclonal antibody (Clone RM320) at a 1:250 dilution.
Specific Publications For Anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal Antibody, Clone#RM320 (M00109-1)
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal Antibody, Clone#RM320
Question
We bought anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
I viewed the website and datasheets of our anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2020-02-21
Question
Is this M00109-1 anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 reactive to the isotypes of CD274?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The immunogen of M00109-1 anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 is A peptide corresponding to the C-terminus of human PD-L1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-27
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for gastric carcinoma using anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 M00109-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-05
Question
Our lab want to know about using your anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 for toxin transport studies. Has this antibody been tested with western blotting on human tonsil tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
I appreciate your inquiry. This M00109-1 anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 is tested on lncap cells, human tonsil tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-30
Question
We have observed staining in human chorionic villus. Do you have any suggestions? Is anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 supposed to stain chorionic villus positively?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
Based on literature chorionic villus does express CD274. Based on Uniprot.org, CD274 is expressed in chorionic villus, placenta, gastric carcinoma, lung, liver, among other tissues. Regarding which tissues have CD274 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Placenta, Pubmed ID: 11015443, 14702039
Boster Scientific Support
Answered: 2019-07-08
Question
See below the WB image, lot number and protocol we used for gastric carcinoma using anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 M00109-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-05-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-30
Question
I am looking for to test anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 M00109-1 on human gastric carcinoma for research purposes, then I may be interested in using anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 M00109-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-03-30
Answer
The products we sell, including anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 M00109-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-30
Question
Would anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 M00109-1 work for IHC with gastric carcinoma?
Verified Customer
Verified customer
Asked: 2017-11-03
Answer
According to the expression profile of gastric carcinoma, CD274 is highly expressed in gastric carcinoma. So, it is likely that anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 M00109-1 will work for IHC with gastric carcinoma.
Boster Scientific Support
Answered: 2017-11-03
Question
My colleagues were well pleased with the WB result of your anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone
B. Walker
Verified customer
Asked: 2016-07-07
Answer
From literature, lung does express CD274. Generally CD274 expresses in cell membrane. Regarding which tissues have CD274 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Placenta, Pubmed ID: 11015443, 14702039
Boster Scientific Support
Answered: 2016-07-07
Question
I was wanting to use your anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 for IHC for human gastric carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human gastric carcinoma identification?
C. Thomas
Verified customer
Asked: 2015-08-31
Answer
As indicated on the product datasheet, M00109-1 anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human gastric carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-08-31
Question
We are currently using anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 M00109-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
R. Krishna
Verified customer
Asked: 2013-06-18
Answer
The anti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal antibody, Clone#RM320 (M00109-1) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-18
