Anti-Phospho NFAT5 (Ser1197) Antibody

NFAT5 antibody

Boster Bio Anti-Phospho NFAT5 (Ser1197) Antibody (Catalog# P01815). Tested in IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: P01815
Size: 100ul
Reactive Species: Human, Mouse
Host: Rabbit
Application: ELISA, IF, ICC

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Product Name

Anti-Phospho NFAT5 (Ser1197) Antibody

View all NFAT5 Antibodies

SKU/Catalog Number

P01815

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Phospho NFAT5 (Ser1197) Antibody (Catalog# P01815). Tested in IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho NFAT5 (Ser1197) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01815)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human NFAT5 around the phosphorylation site of Ser1197. Immunogen sequence location: 1171-1220

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P01815 is reactive to NFAT5 in Human, Mouse

Reconstitution

Observed Molecular Weight

72 kDa

Calculated molecular weight

165763 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

P01815 is guaranteed for ELISA, IF, ICC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunofluorescence, 1:200 - 1:1000
ELISA, 1:10000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For NFAT5 (Source: Uniprot.org, NCBI)

Gene Name

NFAT5

Full Name

Nuclear factor of activated T-cells 5

Weight

165763 MW

Alternative Names

NFAT5;KIAA0827;TONEBP;Nuclear factor of activated T-cells 5;NF-AT5;T-cell transcription factor NFAT5;Tonicity-responsive enhancer-binding protein;TonE-binding protein;TonEBP NFAT5 NF-AT5, NFATL1, NFATZ, OREBP, TONEBP nuclear factor of activated T cells 5 nuclear factor of activated T-cells 5|NFAT-like protein 1|T-cell transcription factor NFAT5|TonE-binding protein|glutamine rich protein H65|nuclear factor of activated T-cells 5, tonicity-responsive|osmotic response element-binding protein|tonicity-responsive enhancer-binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NFAT5, check out the NFAT5 Infographic

NFAT5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NFAT5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-Phospho NFAT5 (Ser1197) Antibody

Question

I would like to test anti-Phospho NFAT5 (Ser1197) antibody P01815 on human kidney for research purposes, then I may be interested in using anti-Phospho NFAT5 (Ser1197) antibody P01815 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-03-06

Answer

The products we sell, including anti-Phospho NFAT5 (Ser1197) antibody P01815, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-03-06

Question

We are currently using anti-Phospho NFAT5 (Ser1197) antibody P01815 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-03

Answer

The anti-Phospho NFAT5 (Ser1197) antibody (P01815) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-03

Question

Is there a BSA free version of anti-Phospho NFAT5 (Ser1197) antibody P01815 available?

Verified Customer

Verified customer

Asked: 2020-01-27

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho NFAT5 (Ser1197) antibody P01815 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-27

Question

Does anti-Phospho NFAT5 (Ser1197) antibody P01815 work on horse IF with kidney?

Verified Customer

Verified customer

Asked: 2018-12-18

Answer

Our lab technicians have not tested anti-Phospho NFAT5 (Ser1197) antibody P01815 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Phospho NFAT5 (Ser1197) antibody P01815 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse kidney in IF, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-12-18

Question

I see that the anti-Phospho NFAT5 (Ser1197) antibody P01815 works with IF, what is the protocol used to produce the result images on the product page?

K. Singh

Verified customer

Asked: 2017-10-09

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-10-09

Question

I was wanting to use your anti-Phospho NFAT5 (Ser1197) antibody for IF for human kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human kidney identification?

P. Thomas

Verified customer

Asked: 2016-03-30

Answer

As indicated on the product datasheet, P01815 anti-Phospho NFAT5 (Ser1197) antibody has been tested for IF on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-03-30

Order DetailsPrice
P01815

100 μL

$335

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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