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Product Info Summary
| SKU: | A06012 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-POLG2 Antibody
SKU/Catalog Number
A06012
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-POLG2 Antibody (Catalog # A06012). Tested in IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-POLG2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06012)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human POLG2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06012 is reactive to POLG2 in Human, Mouse
Applications
A06012 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
54.911kDa
Background of POLG2
Mitochondrial polymerase processivity subunit. Stimulates the polymerase and exonuclease activities, and increases the processivity of the enzyme. Binds to ss-DNA.
Lim S.E., J. Biol. Chem. 274:38197-38203(1999).
Johnson A.A., Biochemistry 39:1702-1708(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue using POLG2 antibody A06012.
Protein Target Info & Infographic
Gene/Protein Information For POLG2 (Source: Uniprot.org, NCBI)
Gene Name
POLG2
Full Name
DNA polymerase subunit gamma-2, mitochondrial
Weight
54.911kDa
Alternative Names
DNA polymerase gamma accessory 55 kDa subunit; DNA polymerase subunit gamma-2, mitochondrial; EC 2.7.7.7; HP55; Mitochondrial DNA polymerase accessory subunit; mitochondrial DNA polymerase subunit gamma-2; mitochondrial DNA polymerase, accessory subunit; MtPolB; MTPOLBPOLG-BETA; p55; PEOA4; POLB; POLGB; PolG-beta; polymerase (DNA directed), gamma 2, accessory subunit POLG2 HP55, MTDPS16, MTPOLB, PEOA4, POLB, POLG-BETA, POLGB DNA polymerase gamma 2, accessory subunit DNA polymerase subunit gamma-2, mitochondrial|DNA polymerase gamma accessory 55 kDa subunit|mitochondrial DNA polymerase subunit gamma-2|mitochondrial DNA polymerase, accessory subunit|p55|polymerase (DNA directed), gamma 2, accessory subunit|polymerase (DNA) gamma 2, accessory subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLG2, check out the POLG2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POLG2 Antibody (A06012)
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No publications found for A06012
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-POLG2 Antibody
Question
We are currently using anti-POLG2 antibody A06012 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on goat tissues as well?
K. Krishna
Verified customer
Asked: 2020-03-13
Answer
The anti-POLG2 antibody (A06012) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-13
Question
Is there a BSA free version of anti-POLG2 antibody A06012 available?
Verified Customer
Verified customer
Asked: 2019-02-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-POLG2 antibody A06012 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-02-20
Question
I was wanting to use your anti-POLG2 antibody for IHC for human cerebellum on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cerebellum identification?
H. Kulkarni
Verified customer
Asked: 2016-12-30
Answer
As indicated on the product datasheet, A06012 anti-POLG2 antibody has been tested for IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human cerebellum in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-12-30
