Anti-DNA-binding protein RFX5 RFX5 Antibody

Product Name

Anti-DNA-binding protein RFX5 RFX5 Antibody

View all RFX5 Antibodies

SKU/Catalog Number

A05527

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-DNA-binding protein RFX5 RFX5 Antibody (Catalog # A05527). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-DNA-binding protein RFX5 RFX5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05527)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

RFX5 (N-terminal specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A05527 is reactive to RFX5 in Human

Observed Molecular Weight

39 kDa

Calculated molecular weight

65.3 kDa

Background of RFX5

DNA-binding protein RFX5 activates transcription from class II MHC promoters and recognizes X-boxes. It mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters. RFX5 may be associated with Bare Lymphocyte Syndrome Type li Complementation group C and Mhc Class Ii Deficiency. Anti-RFX5 Antibody is useful for researchers interested in Transcription factors, Tuberculosis, and Allograft rejection.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05527 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:25,000
WB: 1:500 - 1:3,000
Anti-RFX5 has been tested in western blot. This product was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 µl per assay. 

Validation Images & Assay Conditions

See full target information RFX5

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