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Product Info Summary
| SKU: | PA1071 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-Secretogranin 3/SCG3 Antibody Picoband®
SKU/Catalog Number
PA1071
BA2378 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Secretogranin 3/SCG3 Antibody catalog # PA1071. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Secretogranin 3/SCG3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1071)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Secretogranin 3, different from the related mouse and rat sequences by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1071 is reactive to SCG3 in Human, Mouse, Rat
Observed Molecular Weight
58-60 kDa
Calculated molecular weight
53.0 kDa
Background of SCG3
SCG3 (secretogranin III) is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Genetic variations in the SCG3 gene may influence the risk of obesity through possible regulation of hypothalamic neuropeptide secretion. SCG3 was the only gene within a haplotype block that contained rs3764220. SCG3 mRNA and immunoreactivity were detected in the paraventricular nucleus, lateral hypothalamic area, and arcuate nucleus, and the protein coexisted with orexin, melanin-concentrating hormone, neuropeptide Y, and proopiomelanocortin. SCG3 formed a granule-like structure together with these neuropeptides.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1071 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Rat, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: human SH-SY5Y whole cell, human K562 whole cell, human THP-1 whole cell, human 293T whole cell, rat brain tissue, mouse brain tissue
IHC: Rat Brain Tissue, Human Rectal Cancer Tissue
Validation Images & Assay Conditions
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Western blot analysis of SCG3 using anti-SCG3 antibody (PA1071).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCG3 antigen affinity purified polyclonal antibody (PA1071) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SCG3 at approximately 58-60 kDa. The expected band size for SCG3 is at 53 kDa.
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Anti-Secretogranin 3 antibody, PA1071, IHC(P)
IHC(P): Rat Brain Tissue
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Anti-Secretogranin 3 antibody, PA1071, IHC(P)
IHC(P): Human Rectal Cancer Tissue
Specific Publications For Anti-Secretogranin 3/SCG3 Antibody Picoband® (PA1071)
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Customer Q&As
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7 Customer Q&As for Anti-Secretogranin 3/SCG3 Antibody Picoband®
Question
Would anti-Secretogranin 3/SCG3 antibody PA1071 work for WB with brain lung?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
According to the expression profile of brain lung, SCG3 is highly expressed in brain lung. So, it is likely that anti-Secretogranin 3/SCG3 antibody PA1071 will work for WB with brain lung.
Boster Scientific Support
Answered: 2020-03-25
Question
I was wanting to use your anti-Secretogranin 3/SCG3 antibody for WB for rat brain lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain lung identification?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
It shows on the product datasheet, PA1071 anti-Secretogranin 3/SCG3 antibody has been validated for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-14
Question
Will PA1071 anti-Secretogranin 3/SCG3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
You can see on the product datasheet, PA1071 anti-Secretogranin 3/SCG3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain lung using anti-Secretogranin 3/SCG3 antibody PA1071. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-06
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-06
Question
Is a blocking peptide available for product anti-Secretogranin 3/SCG3 antibody (PA1071)?
Verified Customer
Verified customer
Asked: 2018-01-16
Answer
We do provide the blocking peptide for product anti-Secretogranin 3/SCG3 antibody (PA1071). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-01-16
Question
We are currently using anti-Secretogranin 3/SCG3 antibody PA1071 for rat tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
T. Johnson
Verified customer
Asked: 2016-03-07
Answer
The anti-Secretogranin 3/SCG3 antibody (PA1071) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-03-07
Question
Is this PA1071 anti-Secretogranin 3/SCG3 antibody reactive to the isotypes of SCG3?
S. Johnson
Verified customer
Asked: 2016-01-15
Answer
The immunogen of PA1071 anti-Secretogranin 3/SCG3 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Secretogranin 3(438-450aa DFINKQADAYVEK), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-01-15
