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Product Info Summary
| SKU: | M02318 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-PGM1 Antibody (C-Term)
SKU/Catalog Number
M02318
Size
80 µl
Description
Boster Bio Anti-PGM1 Antibody (C-Term) (Catalog # M02318). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PGM1 Antibody (C-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02318)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This PGM1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 469-501 amino acids from human PGM1.
Reactive Species
M02318 is reactive to PGM1 in Human, Mouse
Calculated molecular weight
61.4 kDa
Background of PGM1
This enzyme participates in both the breakdown and synthesis of glucose.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02318 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-PGM1 Antibody (C-Term) at 1:2000 dilution
Lane 1: human liver lysate
Lane 2: mouse heart lysate
Lane 3: Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 61 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-PGM1 Antibody (C-Term) at 1:2000 dilution
Lane 1: mouse heart lysates
Lane 2: Jurkat whole cell lysates
Lane 3: human liver lysates
Lane 4: NIH/3T3 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 61 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
All lanes : Anti-PGM1 Antibody (C-Term) at 1:2000 dilution
Lane 1: mouse brain lysates
Lane 2: mouse heart lysates
Lane 3: human liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 61 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M02318 staining PGM1 in human heart tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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M02318 staining PGM1 in human liver tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-PGM1 Antibody (C-Term) (M02318)
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