Mouse PLA2G2A ELISA Kit PicoKine®

Pla2g2a ELISA kit for Mouse

Mouse PLA2G2A ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Pla2g2a in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK1945
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Mouse PLA2G2A ELISA Kit PicoKine®

View all Pla2g2a ELISA kits

SKU/Catalog Number

EK1945

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse PLA2G2A ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Pla2g2a in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse PLA2G2A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1945)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: N22-C146

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1945 is reactive to Pla2g2a in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1945 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Pla2g2a

Phospholipase A2, membrane associated is an enzyme that in humans is encoded by the PLA2G2A gene. It is mapped to 4 D3; 4 70.57 cM. Proteins belonging to the phospholipase A2 (PLA2) family hydrolyze phospholipids into sn2 fatty acids and lysophospholipids. They function in a variety of cellular processes, including the digestion of phospholipids and the production of molecules that induce inflammatory responses. This gene encodes a member of the group II class of secretory PLA2s. The secreted enzyme binds to heparin on the cell surface. Mutations in this gene increase the occurrence of intestinal polyps caused by a dominant mutation in the adenomatosis polyposis coli gene. A frameshift inactivates this gene product in some mouse strains including the strain of the reference genome, C57BL/6J, whereas a functional protein is produced in other strains.

Kit Components

Catalog Number Description Quantity
EK1945-CAP Anti-Mouse Pla2g2a Pre-coated 96-well strip microplate 1
EK1945-ST Mouse Pla2g2a Standard 2 vials, 10 ng/tube
EK1945-DA Mouse Pla2g2a Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0510.1850.3120.5150.8241.1421.4481.722

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Pla2g2a, Dilution ratio of 1:1, concentration in serum is around 400pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse PLA2G2A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5623493858242951
Standard deviation2.6913.3443.152.9614.2860.86
CV (%)4.8%5.7%4.6%5.1%5.9%6.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Pla2g2a in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 156595562582.734.7%
Sample 22342322342342330.860.3%
Sample 393898310551031100145.014.4%
*number of samples for each test n=16.

Gene/Protein Information For Pla2g2a (Source: Uniprot.Org, NCBI)

Uniprot ID

P31482

Gene ID

18780

Gene Name

Pla2g2a

Full Name

Phospholipase A2, membrane associated

Weight

16.145kDa

Superfamily

phospholipase A2 family

Alternative Names

EC 3.1.1.4; GIIC sPLA2; Group IIA phospholipase A2; Mom1; Non-pancreatic secretory phospholipase A2; NPS-PLA2; Phosphatidylcholine 2-acylhydrolase 2A; phospholipase A2, group IIA (platelets, synovial fluid); phospholipase A2, membrane associated; PLA2B; PLA2BPLA2; PLA2G2A; PLA2L; PLA2LPLA2S; PLAS1; RASF-A; sPLA2 Pla2g2a|EF, Mom1, Pla2, sPLA2, sPla2-IIA|phospholipase A2, group IIA (platelets, synovial fluid)|phospholipase A2, membrane associated|GIIC sPLA2|enhancing factor|group IIA phospholipase A2|modifier of Min1|non-pancreatic secreted type II phospholipase A2|phosphatidylcholine 2-acylhydrolase 2A|secretory group II phospholipase A2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Pla2g2a, check out the Pla2g2a Infographic

Pla2g2a infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Pla2g2a: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1945 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Moslea Herba flavonoids alleviated influenza A virus-induced pulmonary endothelial barrier disruption via suppressing NOX4/NF-κB/MLCK pathway

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8 Customer Q&As for Mouse PLA2G2A ELISA Kit PicoKine®

Question

We intend to use mouse serum of C57BL/6 as a negative control. Do you think EK1945 cannot recognize mouse strains C57BL/6?

Verified customer

Asked: 2021-01-06

Answer

The immunogen sequence of both the capture antibody and detection antibody is N22-C146. Our lab has never tested mouse serum of C57BL/6 using the Mouse PLA2G2A ELISA Kit PicoKine™ (EK1945) and we are uncertain if the sample could be used as a negative control. We suggest to run a pilot test to see if PLA2G2A could be tested in mouse serum of C57BL/6.

Boster Scientific Support

Answered: 2021-01-06

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis PLA2G2A using the EK1945 Mouse PLA2G2A Picokine® ELISA Kit?

K. Turner

Verified customer

Asked: 2020-05-06

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2020-05-06

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-12-03

Question

Q: Can PLA2G2A ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

K. Hall

Verified customer

Asked: 2019-10-22

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-10-22

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-07-28

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-07-28

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

J. Mitchell

Verified customer

Asked: 2018-06-24

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-06-24

Question

Q: how to analyze ELISA data? I measured PLA2G2A level in serum.

G. Singh

Verified customer

Asked: 2018-05-26

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-05-26

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

L. Bhasin

Verified customer

Asked: 2017-04-10

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2017-04-10

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