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Product Info Summary
| SKU: | EK1533 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human GREM2/Prdc ELISA Kit PicoKine®
View all PRDC/GREM2 ELISA kits
SKU/Catalog Number
EK1533
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human GREM2/Prdc ELISA Kit PicoKine™ (96 Tests). Quantitate Human GREM2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human GREM2/Prdc ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1533)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: R22-Q168
Sensitivity
<12 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1533 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1533 is reactive to Grem2 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1533 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PRDC/GREM2
GREM2, also known as PRDC, is one of several BMP antagonists that regulate the functions of BMPs during embryonic development and tissue differentiation. BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. The PRDC gene is mapped to chromosome 1q43.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1533-CAP | Anti-Human Grem2 Pre-coated 96-well strip microplate | 1 |
| EK1533-ST | Human Grem2 Standard | 2 vials, 10 ng/tube |
| EK1533-DA | Human Grem2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.090 | 0.171 | 0.211 | 0.345 | 0.532 | 0.890 | 1.558 | 2.342 |
Data Example Images
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Human GREM2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect PRDC/GREM2, Dilution ratio of 1:1, concentration in serum and plasma is around 700 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human GREM2/Prdc ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 142 | 522 | 1488 | 140 | 556 | 1359 |
| Standard deviation | 10.08 | 39.15 | 113.08 | 12.32 | 45.03 | 107.36 |
| CV (%) | 7.1% | 7.5% | 7.6% | 8.8% | 8.1% | 7.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of PRDC/GREM2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 142 | 139 | 149 | 126 | 139 | 8.33 | 5.9% |
| Sample 2 | 522 | 566 | 575 | 538 | 550 | 21.26 | 3.8% |
| Sample 3 | 1488 | 1697 | 1588 | 1757 | 1632 | 103.1 | 6.3% |
Protein Target Info & Infographic
Gene/Protein Information For Grem2 (Source: Uniprot.Org, NCBI)
Gene Name
Grem2
Full Name
Gremlin-2
Weight
19.334kDa
Superfamily
DAN family
Alternative Names
CKTSF1B2; CKTSF1B2FLJ21195; Cysteine knot superfamily 1, BMP antagonist 2; DAN domain family member 3; DAND3; DAND3Prdc; GREM2; gremlin 2; gremlin 2, cysteine knot superfamily, homolog; gremlin-2; PRDC; PRDCgremlin 2, cysteine knot superfamily, homolog (Xenopus laevis); Protein related to DAN and cerberus Grem2|Gremlin2, Pr, Prdc|gremlin 2, DAN family BMP antagonist|gremlin-2|Gremlin1|cysteine knot superfamily 1, BMP antagonist 2|gremlin 2 homolog, cysteine knot superfamily|protein related to DAN and cerberus
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Grem2, check out the Grem2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Grem2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human GREM2/Prdc ELISA Kit PicoKine® (EK1533)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Human GREM2/Prdc ELISA Kit PicoKine®
Question
Q: can you suggest the dilution ratio of serum samples for detection of PRDC in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-09-02
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the PRDC ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-09-02
Question
Q: how can I thaw whole blood sample for PRDC ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test PRDC for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-11-05
Question
Q: can I use citrate plasma as samples in Human PRDC Picokine® ELISA Kit (Catalog # EK1533)?
Verified Customer
Verified customer
Asked: 2019-04-12
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of PRDC causing degradation of its protein structure. PRDC may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the PRDC ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-04-12
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-11-28
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-11-28
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification PRDC using the EK1533 Human PRDC Picokine® ELISA Kit?
K. King
Verified customer
Asked: 2018-06-10
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2018-06-10
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-05-01
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-05-01
Question
Q: how do I analyze ELISA data? I measured PRDC level in serum.
Verified Customer
Verified customer
Asked: 2017-06-07
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-06-07
