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Product Info Summary
| SKU: | A06326-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SKA2 Antibody
SKU/Catalog Number
A06326-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SKA2 Antibody (Catalog # A06326-1). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
SKA2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SKA2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06326-1)
Host
Rabbit
Contents
SKA2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SKA2 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human SKA2. The immunogen is located within the first 50 amino acids of SKA2.
Reactive Species
A06326-1 is reactive to SKA2 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
14.2 kDa
Background of SKA2
Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing. SKA2 has also been shown to interact with glucocorticoid receptors and to be involved in glucocorticoid signaling and cell proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06326-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SKA2 antibody can be used for detection of SKA2 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SKA2 in 3T3 cell lysate with SKA2 antibody at (A) 0.5 and (B) 1 μg/mL.
Specific Publications For Anti-SKA2 Antibody (A06326-1)
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