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Product Info Summary
| SKU: | A00745S126-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-Cyclin B1 (S126) CCNB1 Antibody
SKU/Catalog Number
A00745S126-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Cyclin B1 (S126) CCNB1 Antibody catalog # A00745S126-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Cyclin B1 (S126) CCNB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00745S126-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Cyclin B1 around the phosphorylation site of Ser126. AA range:91-140
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00745S126-1 is reactive to CCNB1 in Human, Mouse, Rat
Calculated molecular weight
48.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00745S126-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Phospho-Cyclin B1 (S126) Polyclonal Antibody
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Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 15', using Cyclin B1 (Phospho-Ser126) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Cyclin B1 (Phospho-Ser126) Antibody. The picture on the right is blocked with the phospho peptide.
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Cyclin B1 (Phospho-Ser126) Antibody
Specific Publications For Anti-Phospho-Cyclin B1 (S126) CCNB1 Antibody (A00745S126-1)
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