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Product Info Summary
| SKU: | M30952 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | All species |
| Host: | Mouse |
| Application: | ELISA, IHC, RIA |
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Product info
Product Name
Anti-Testosterone Monoclonal Antibody
SKU/Catalog Number
M30952
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Testosterone Monoclonal Antibody (Catalog # M30952). Tested in ELISA, RIA, IHC applications. This antibody reacts with All species.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Testosterone Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30952)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: 4E1G2
Isotype
IgG1, kappa
Immunogen
Testosterone 3 CMO conjugated to BSA
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M30952 is reactive to in All species
Applications
M30952 is guaranteed for ELISA, IHC, RIA Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
29159 MW
Background of
This monoclonal antibody is highly specific to testosterone. Its affinity constant for testosterone is ~1010M-1. In competitive binding immunoassay, it reacts with testosterone 100%, 11-beta-hydroxy testosterone 3.3%, 17-alpha-methyl testosterone
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA (For coating, order antibody without BSA)
Radioimmunoassay (RIA)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Anti-Testosterone Mouse Monoclonal Antibody (4E1G2).
Specific Publications For Anti-Testosterone Monoclonal Antibody (M30952)
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