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Product Info Summary
| SKU: | LS200558 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of aprataxin and PNKP like factor (APLF) |
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Product info
Product Name
APLF (NM_173545) Human Over-expression Lysate
SKU/Catalog Number
LS200558
Size
100 µg
Description
Transient overexpression lysate of aprataxin and PNKP like factor (APLF)
Storage & Handling
Cite This Product
APLF (NM_173545) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS200558)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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APLF Human Over-expression Lysates NM_173545.
Western blot validation of overexpression lysate (LS200558) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with APLF (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for Aplf (Source: Uniprot.org, NCBI)
Gene Name
Aplf
Full Name
Aprataxin and PNK-like factor
Weight
54.968kDa
Superfamily
APLF family
Alternative Names
APFL; aprataxin- and PNK-like factor; aprataxin and PNK-like factor; aprataxin and PNKP like factor; Apurinic-apyrimidinic endonuclease APLF; C2orf13FLJ16593; chromosome 2 open reading frame 13; EC 4.2.99.18; MGC47799; PALF; PNK and APTX-like FHA domain-containing protein; PNK and APTX-like FHA protein; Xip1; XRCC1-interacting protein 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on Aplf, check out the Aplf Infographic
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In this infographic, you will see the following information for Aplf: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For APLF (NM_173545) Human Over-expression Lysate (LS200558)
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