Product Info Summary
SKU: | LS009296 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F (ATP6V1F) |
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Product info
Product Name
ATP6V1F (NM_004231) Human Over-expression Lysate
See all V-type proton ATPase subunit F products
SKU/Catalog Number
LS009296
Size
100 µg
Description
Transient overexpression lysate of ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F (ATP6V1F)
Storage & Handling
Cite This Product
ATP6V1F (NM_004231) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS009296)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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ATP6V1F Human Over-expression Lysates NM_004231.
Western blot validation of overexpression lysate (LS009296) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ATP6V1F (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ATP6V1F (Source: Uniprot.org, NCBI)
Gene Name
ATP6V1F
Full Name
V-type proton ATPase subunit F
Weight
13.37kDa
Superfamily
V-ATPase F subunit family
Alternative Names
ATP6S14adenosinetriphosphatase 14k chain; ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F; H(+)-transporting two-sector ATPase, 14kD subunit; MGC117321; MGC126037; MGC126038; vacuolar ATP synthase subunit F; vacuolar proton pump F subunit; Vacuolar proton pump subunit F; VATFATPase, vacuolar, 14 kD; V-ATPase 14 kDa subunit; V-ATPase F subunit; V-ATPase subunit F; Vma7; V-type proton ATPase subunit F ATP6V1F ATP6S14, VATF, Vma7 ATPase H+ transporting V1 subunit F V-type proton ATPase subunit F|ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F|ATPase, vacuolar, 14 kD|H(+)-transporting two-sector ATPase, 14kD subunit|V-ATPase 14 kDa subunit|V-ATPase F subunit|V-ATPase subunit F|adenosinetriphosphatase 14k chain|vacuolar ATP synthase subunit F|vacuolar proton pump F subunit|vacuolar proton pump subunit F
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ATP6V1F, check out the ATP6V1F Infographic
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In this infographic, you will see the following information for ATP6V1F: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ATP6V1F (NM_004231) Human Over-expression Lysate (LS009296)
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