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Product Info Summary
| SKU: | LS000657 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of bone morphogenetic protein receptor, type IA (BMPR1A) |
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Product info
Product Name
BMPR1A (NM_004329) Human Over-expression Lysate
See all BMPR-IA/ALK-3 products
SKU/Catalog Number
LS000657
Size
100 µg
Description
Transient overexpression lysate of bone morphogenetic protein receptor, type IA (BMPR1A)
Storage & Handling
Cite This Product
BMPR1A (NM_004329) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS000657)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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BMPR1A Human Over-expression Lysates NM_004329.
Western blot validation of overexpression lysate (LS000657) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with BMPR1A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for BMPR1A (Source: Uniprot.org, NCBI)
Gene Name
BMPR1A
Full Name
Bone morphogenetic protein receptor type-1A
Weight
60.198kDa
Superfamily
protein kinase superfamily
Alternative Names
ACVRLK310q23del; ALK-3; ALK3EC 2.7.11.30; BMP type-1A receptor; BMPR1A; BMPR-1A; BMPRIA; BMPR-IA; bone morphogenetic protein receptor type-1A; bone morphogenetic protein receptor, type IA; CD292 antigen; CD292; EC 2.7.11; Serine/threonine-protein kinase receptor R5; SKR5; type II-like kinase 3 BMPR1A 10q23del, ACVRLK3, ALK3, CD292, SKR5 bone morphogenetic protein receptor type 1A bone morphogenetic protein receptor type-1A|ALK-3|BMP type-1A receptor|BMPR-1A|activin A receptor, type II-like kinase 3|activin receptor-like kinase 3|bone morphogenetic protein receptor, type IA|serine/threonine-protein kinase receptor R5
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on BMPR1A, check out the BMPR1A Infographic
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In this infographic, you will see the following information for BMPR1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For BMPR1A (NM_004329) Human Over-expression Lysate (LS000657)
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No publications found for LS000657
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