Transcription factor AP-1 Antibodies

Transcription factor AP-1 (JUN)

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes action of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.

Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).

Gene Information

Human
Gene Name:	JUN
Uniprot:	P05412
Entrez:	3725

Family

Belongs to:
bZIP family

Alternative Names

Activator protein 1; AP1; AP-1; cJun; c-Jun; enhancer-binding protein AP1; Jun activation domain binding protein; jun oncogene; jun proto-oncogene; JUN; Proto-oncogene c-Jun; transcription factor AP-1; V-jun avian sarcoma virus 17 oncogene homologp39; v-jun sarcoma virus 17 oncogene homolog (avian); v-jun sarcoma virus 17 oncogene homolog

Molecular Weight

Mass (kDA):
35.676 kDA

Genomic Context

Human
Location:	1p32.1
Sequence:	1; NC_000001.11 (58780791..58784047, complement)

Subcellular Localizations

Nucleus.

Diseases

• Neoplasms
• Malignant Neoplasms
• Cell Transformation, Neoplastic
• Carcinoma
• Inflammation
• Malignant Paraganglionic Neoplasm
• Leukemia
• Hiv Infections
• Mammary Neoplasms
• Malignant Neoplasm Of Breast
• Infective Disorder
• Immunologic Deficiency Syndromes
• Neoplasm Metastasis

• Liver Carcinoma
• Carcinogenesis
• Nervousness
• Acquired Immunodeficiency Syndrome
• Hyperplasia
• Tissue Adhesions
• Metaplastic Cell Transformation

Interacting Proteins

• APLP2
• APP
• ATF1
• ATF2
• ATF3

• ATF4
• ATF7
• BATF
• BATF3
• BBS7

• CITED1
• CREB3
• ETS1
• FOS
• FOSL1

• FOSL2
• GOPC
• HSP90AA1
• HSPA8
• KPNA2

• MAPK10
• MAPK8
• MAPRE3
• Maz
• MDM2

• MTA1
• NEFM
• NFATC1
• PRRC2A
• SMAD2

• STRN4
• TCL1A
• TUBA1A
• TUBB
• VRK1

Pathways

• Cell Proliferation
• Cell Death
• Cell Cycle
• Cell Growth
• Localization
• Secretion
• Pathogenesis
• Regeneration
• Cell Differentiation
• Translation
• Immune Response
• Programmed Cell Death
• Cell Activation

• Oncogenesis
• Dna Repair
• Induction Of Apoptosis
• Inflammatory Response
• Transport
• Regulation Of Gene Expression
• Angiogenesis

PTMs

• Phosphorylation
• Cleavage
• Dephosphorylation
• Ubiquitination
• Reduction
• Oxidation
• Methylation
• Acetylation
• Phosphorothioation
• Sumoylation

• Biotinylation
• Glycosylation
• Farnesylation
• Deacetylation
• Geranylgeranylation
• Myristoylation
• Sulphation
• Demethylation
• Ribosylation
• Deamidation

Research Areas

• Apoptosis
• Cancer
• Cell Cycle and Replication
• Hypoxia
• Immunology

• Innate Immunity
• Signal Transduction
• Transcription Factors and Regulators
• Tumor Suppressors
• Wnt Signaling Pathway

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/JUN

Boster Bio - Best Uses Of The JUN Marker

In this article, we'll look at the benefits of Immunofluorescence-based assays for the JUN Marker, a Purified Polyclonal Antibody from Boster Bio. This antibody has the potential to help researchers better understand the role of this protein in cell signaling. However, the process can be challenging. Fortunately, the Boster Bio system comes with helpful guides to help you use the system in the best way possible.

Boster Bio: Purified Polyclonal Antibody

Using the Boster Bio: Purified PolyclonAL Antibody for the JUN Marker, researchers can test whether their sample reacts with the JUN protein on a variety of cancer cells. The antibodies react with Human, Mouse and Rat proteins, making them suitable for use in a range of applications. Boster Bio: Purified Polyclonal Antibody for the JUN Marker is available in a variety of formats to suit your needs.

This monoclonal antibody is supplied in PBS containing 0.09% sodium azide, and is purified by peptide affinity purification and a protein A column. The antibody is produced from rabbits immunized with a synthetic peptide (KLH-conjugated). To perform a test, you must have a blocking peptide for JUN before purchasing a polyclonal antibody.

Monoclonal antibodies were developed by Cesar Milstein and Georges Kohler, whose discoveries helped pave the way for rational design of antibodies. This method was soon adopted and has revolutionized antibody research. The focus has shifted from hijacking adaptive immune systems to rational design of antibodies. Increasing the rate of discovery and development of antibodies has changed the way we treat cancer.

These antibodies have been successfully tested in several clinical trials. They have been able to recognize a variety of recombinant proteins in animal cell lines, including the coat protein of the Pelargonium zonate spot virus. These antibodies are also capable of detecting GFP. The quality of these antibodies is assessed by a series of western blot assays.

The purified monoclonal anti-NOS-3 antibody has been produced using goat IgG. It is purified using affinity chromatography. It was tested in 13 different clinical trials and is cited in over a hundred publications. The monoclonal antibody is also highly specific to mouse IgG. So it is the best choice for immunostaining cells.

To produce monoclonal antibodies for a phosphorylated protein, one must perform several affinity chromatography steps. First, crude antiserum is passed over a phospho-peptide column. After that, it is washed to remove low-complexity epitopes. This process requires a significant amount of time and can be unsuccessful.

This monoclonal antibody recognizes the JUN protein as a partner of a p38-p53 complex. It promotes the transcriptional activity of the JUN protein by inhibiting ubiquitination-mediated degradation. Furthermore, Boster Bio: Purified Polyclonal Antibody for the JUN Marker

This antibody is made with the primary-secondary antibody system, which enables dual labeling of specimens. This allows researchers to ask more questions about the specimens using the same antibodies, thus yielding more robust answers and contextual data. These antibodies are also highly specific, enabling researchers to detect the JUN Marker with high accuracy. And they are affordable. But be careful, it is important to make the right choice.

Boster Bio: Immunofluorescence

The JUN gene is a putative transforming gene that interacts with target DNA sequences to regulate gene expression. It is intronless, and it maps to 1p32-p31, which is involved in the translocation and deletion of many human malignancies. All Boster antibodies have been validated for use on WB and IHC. This means that they are high-quality and can be trusted by scientists worldwide.

When c-Jun is phosphorylated, it stimulates Fasl, which activates Caspase-8, which then motivates the downstream effector Caspase-3 to initiate apoptosis. Similarly, EPS116 increases total c-Jun, as well as Ser63 phosphorylation. c-Jun has been shown to induce apoptosis in different cell lines.