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Product Info Summary
| SKU: | M02038 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-c-Jun Rabbit Monoclonal Antibody
SKU/Catalog Number
M02038
BM4168 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-c-Jun Rabbit Monoclonal Antibody catalog # M02038. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-c-Jun Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02038)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CAO-10
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human c-Jun
Reactive Species
M02038 is reactive to JUN in Human, Mouse, Rat
Observed Molecular Weight
43 kDa
Calculated molecular weight
35.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02038 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: human A431 whole cell, human U87 whole cell, human U2OS whole cell, rat C6 whole cell, mouse Neuro-2a whole cell
IHC: Rat cerebral cortex tissue, Rat cerebellum tissue, Human squamous carcinoma tissue, Human breast cancer tissue, Mouse skeletal muscle - gastrocnemius tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of c-Jun using anti-c-Jun antibody (M02038).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Jun antigen affinity purified monoclonal antibody (Catalog # M02038) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Jun at approximately 43 kDa. The expected band size for c-Jun is at 36 kDa.
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IF analysis of c-Jun using anti-c-Jun antibody (M02038).
c-Jun was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 with rabbit anti-c-Jun Antibody (M02038) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat cerebellum, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human squamous carcinoma, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human breast cancer, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle - gastrocnemius , using the Antibody at 1:500 dilution.
Specific Publications For Anti-c-Jun Rabbit Monoclonal Antibody (M02038)
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3 Customer Q&As for Anti-c-Jun Rabbit Monoclonal Antibody
Question
Here is the WB image, lot number and protocol we used for amniotic fluid using anti-c-Jun Rabbit Monoclonal antibody M02038. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-01
Question
Is a blocking peptide available for product anti-c-Jun Rabbit Monoclonal antibody (M02038)?
S. Krishna
Verified customer
Asked: 2016-02-17
Answer
We do provide the blocking peptide for product anti-c-Jun Rabbit Monoclonal antibody (M02038). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2016-02-17
Question
I have a question about product M02038, anti-c-Jun Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Moore
Verified customer
Asked: 2013-12-16
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02038 anti-c-Jun Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-12-16
