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Product Info Summary
| SKU: | A02038 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-JUN Antibody (C-term)
SKU/Catalog Number
A02038
Size
80 µl
Description
Boster Bio Anti-JUN Antibody (C-term) (Catalog # A02038). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-JUN Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02038)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This JUN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 222-251 amino acids from the C-terminal region of human JUN.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02038 is reactive to JUN in Human
Reconstitution
Calculated molecular weight
35676 Da
Background of c-jun
JUN is the putative transforming gene of avian sarcoma virus 17. JUN is a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02038 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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JUN Antibody (C-term) western blot analysis in U251 cell line lysates (35ug/lane). This demonstrates the JUN antibody detected the JUN protein (arrow).
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JUN Antibody (C-term) (Cat. #A02038) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of JUN Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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JUN Antibody (C-term) (Cat. #A02038) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For JUN (Source: Uniprot.org, NCBI)
Gene Name
JUN
Full Name
Transcription factor AP-1
Weight
35676 Da
Superfamily
bZIP family
Alternative Names
Transcription factor AP-1, Activator protein 1, AP1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, JUN JUN AP-1, AP1, c-Jun, cJUN, p39 Jun proto-oncogene, AP-1 transcription factor subunit transcription factor AP-1|Jun activation domain binding protein|activator protein 1|enhancer-binding protein AP1|jun oncogene|proto-oncogene c-Jun|proto-oncogene cJun|v-jun avian sarcoma virus 17 oncogene homolog|v-jun sarcoma virus 17 oncogene homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on JUN, check out the JUN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for JUN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-JUN Antibody (C-term) (A02038)
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