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Product Info Summary
| SKU: | A02038 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-JUN Antibody (C-term)
SKU/Catalog Number
A02038
Size
80 µl
Description
Boster Bio Anti-JUN Antibody (C-term) (Catalog # A02038). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-JUN Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02038)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This JUN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 222-251 amino acids from the C-terminal region of human JUN.
Reactive Species
A02038 is reactive to JUN in Human
Calculated molecular weight
35.7 kDa
Background of JUN
JUN is the putative transforming gene of avian sarcoma virus 17. JUN is a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02038 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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JUN Antibody (C-term) western blot analysis in U251 cell line lysates (35ug/lane). This demonstrates the JUN antibody detected the JUN protein (arrow).
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JUN Antibody (C-term) (Cat. #A02038) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of JUN Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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JUN Antibody (C-term) (Cat. #A02038) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-JUN Antibody (C-term) (A02038)
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