Product Info Summary
SKU: | LS056672 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of chromosome 11 open reading frame 17 (C11orf17), transcript variant 1 |
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Product info
Product Name
C11orf17 (AKIP1) (NM_182901) Human Over-expression Lysate
SKU/Catalog Number
LS056672
Size
100 µg
Description
Transient overexpression lysate of chromosome 11 open reading frame 17 (C11orf17), transcript variant 1
Storage & Handling
Cite This Product
C11orf17 (AKIP1) (NM_182901) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS056672)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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C11orf17 (AKIP1) Human Over-expression Lysates NM_020642.
Western blot validation of overexpression lysate (LS056672) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with AKIP1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
C11orf17 (AKIP1) Human Over-expression Lysates NM_182901.
Western blot validation of overexpression lysate (LS056672) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with AKIP1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for AKIP1 (Source: Uniprot.org, NCBI)
Gene Name
AKIP1
Full Name
A-kinase-interacting protein 1
Weight
23.114kDa
Alternative Names
AKIP1koyt binding protein 2; BCA3koyt binding protein 3; breast cancer associated gene 3; Breast cancer-associated gene 3 protein; chromosome 11 open reading frame 17; koyt binding protein 1; PKA-interacting protein; proline-rich protein BCA3; protein kinase A-interacting protein 1 AKIP1 BCA3, C11orf17 A-kinase interacting protein 1 A-kinase-interacting protein 1|A kinase (PRKA) interacting protein 1|breast cancer associated gene 3|koyt binding protein 1|koyt binding protein 2|koyt binding protein 3|proline-rich protein BCA3
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on AKIP1, check out the AKIP1 Infographic
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In this infographic, you will see the following information for AKIP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For C11orf17 (AKIP1) (NM_182901) Human Over-expression Lysate (LS056672)
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