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Product Info Summary
| SKU: | A30562 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-BCA3 AKIP1 Antibody
SKU/Catalog Number
A30562
Size
100ul
Form
Liquid
Description
Boster Bio Anti-BCA3 AKIP1 Antibody (Catalog# A30562). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BCA3 AKIP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30562)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human BCA3. Immunogen sequence location: 111-160
Reactive Species
A30562 is reactive to AKIP1 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
23.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30562 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using BCA3 Polyclonal Antibody.
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Immunohistochemistry analysis of paraffin-embedded human heart tissue, using BCA3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from Jurkat cells, using BCA3 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HepG2 cells using BCA3 antibody.
Specific Publications For Anti-BCA3 AKIP1 Antibody (A30562)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-BCA3 AKIP1 Antibody
Question
Is a blocking peptide available for product anti-BCA3 antibody (A30562)?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
We do provide the blocking peptide for product anti-BCA3 antibody (A30562). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-05-06
Question
Can you help my question with product A30562, anti-BCA3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30562 anti-BCA3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-28
Question
My question regards to test anti-BCA3 antibody A30562 on human kidney for research purposes, then I may be interested in using anti-BCA3 antibody A30562 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
The products we sell, including anti-BCA3 antibody A30562, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-09
Question
We are currently using anti-BCA3 antibody A30562 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
The anti-BCA3 antibody (A30562) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-31
Question
Does anti-BCA3 antibody A30562 work on pig IHC with lymph node?
Verified Customer
Verified customer
Asked: 2019-04-11
Answer
Our lab technicians have not validated anti-BCA3 antibody A30562 on pig. You can run a BLAST between pig and the immunogen sequence of anti-BCA3 antibody A30562 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig lymph node in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-11
Question
Would anti-BCA3 antibody A30562 work for WB with kidney?
S. Collins
Verified customer
Asked: 2018-05-09
Answer
According to the expression profile of kidney, AKIP1 is highly expressed in kidney. So, it is likely that anti-BCA3 antibody A30562 will work for WB with kidney.
Boster Scientific Support
Answered: 2018-05-09
