Product Info Summary
SKU: | LS022794 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of cancer susceptibility candidate 3 (CASC3) |
Product info
Product Name
CASC3 (NM_007359) Human Over-expression Lysate
SKU/Catalog Number
LS022794
Size
100 µg
Description
Transient overexpression lysate of cancer susceptibility candidate 3 (CASC3)
Storage & Handling
Cite This Product
CASC3 (NM_007359) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS022794)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
Click image to see more details
CASC3 Human Over-expression Lysates NM_007359.
Western blot validation of overexpression lysate (LS022794) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CASC3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for CASC3 (Source: Uniprot.org, NCBI)
Gene Name
CASC3
Full Name
Protein CASC3
Weight
76.278kDa
Superfamily
CASC3 family
Alternative Names
barentsz; Btz; cancer susceptibility candidate 3; Cancer susceptibility candidate gene 3 protein; metastatic lymph node 51; Metastatic lymph node gene 51 protein; MLN 51; MLN51BTZ; Protein barentsz; protein CASC3 CASC3 BTZ, MLN51 CASC3 exon junction complex subunit protein CASC3|MLN 51|barentsz|cancer susceptibility 3|cancer susceptibility candidate 3|cancer susceptibility candidate gene 3 protein|metastatic lymph node 51|metastatic lymph node gene 51 protein|protein barentsz
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CASC3, check out the CASC3 Infographic
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In this infographic, you will see the following information for CASC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For CASC3 (NM_007359) Human Over-expression Lysate (LS022794)
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No publications found for LS022794
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