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Product Info Summary
| SKU: | LS001452 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of casein kinase 1, alpha 1 (CSNK1A1), transcript variant 1 |
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Product info
Product Name
Casein Kinase 1 alpha (CSNK1A1) (NM_001025105) Human Over-expression Lysate
See all Casein Kinase 1 alpha products
SKU/Catalog Number
LS001452
Size
100 µg
Description
Transient overexpression lysate of casein kinase 1, alpha 1 (CSNK1A1), transcript variant 1
Storage & Handling
Cite This Product
Casein Kinase 1 alpha (CSNK1A1) (NM_001025105) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS001452)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Casein Kinase 1 alpha (CSNK1A1) Human Over-expression Lysates NM_001025105.
Western blot validation of overexpression lysate (LS001452) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CSNK1A1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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Casein Kinase 1 alpha (CSNK1A1) Human Over-expression Lysates NM_001892.
Western blot validation of overexpression lysate (LS001452) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CSNK1A1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for CSNK1A1 (Source: Uniprot.org, NCBI)
Gene Name
CSNK1A1
Full Name
Casein kinase I isoform alpha
Weight
38.915kDa
Superfamily
protein kinase superfamily
Alternative Names
Casein Kinase 1 alpha; casein kinase 1, alpha 1; casein kinase I isoform alpha; CK1CKI-alpha; CSNK1A1; down-regulated in lung cancer; EC 2.7.11.1; HLCDGP1; PRO2975 CSNK1A1 CK1, CK1a, CKIa, HEL-S-77p, HLCDGP1, PRO2975 casein kinase 1 alpha 1 casein kinase I isoform alpha|CKI-alpha|clock regulator kinase|down-regulated in lung cancer|epididymis secretory sperm binding protein Li 77p
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CSNK1A1, check out the CSNK1A1 Infographic
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In this infographic, you will see the following information for CSNK1A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Casein Kinase 1 alpha (CSNK1A1) (NM_001025105) Human Over-expression Lysate (LS001452)
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