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Product Info Summary
| SKU: | LS005754 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of PTK7 protein tyrosine kinase 7 (PTK7), transcript variant PTK7-3 |
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Product info
Product Name
CCK4 (PTK7) (NM_152881) Human Over-expression Lysate
SKU/Catalog Number
LS005754
Size
100 µg
Description
Transient overexpression lysate of PTK7 protein tyrosine kinase 7 (PTK7), transcript variant PTK7-3
Storage & Handling
Cite This Product
CCK4 (PTK7) (NM_152881) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005754)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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CCK4 (PTK7) Human Over-expression Lysates NM_152881.
Western blot validation of overexpression lysate (LS005754) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PTK7 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PTK7 (Source: Uniprot.org, NCBI)
Gene Name
PTK7
Full Name
Inactive tyrosine-protein kinase 7
Weight
118.392kDa
Superfamily
protein kinase superfamily
Alternative Names
CCK4; CCK-4; CCK4Colon carcinoma kinase 4; colon carcinoma kinase-4; Protein-tyrosine kinase 7; Pseudo tyrosine kinase receptor 7; PTK7 protein tyrosine kinase 7; PTK7; Tyrosine-protein kinase-like 7 PTK7 CCK-4, CCK4 protein tyrosine kinase 7 (inactive) inactive tyrosine-protein kinase 7|PTK7 protein tyrosine kinase 7|colon carcinoma kinase 4|pseudo tyrosine kinase receptor 7|tyrosine-protein kinase-like 7
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PTK7, check out the PTK7 Infographic
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In this infographic, you will see the following information for PTK7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For CCK4 (PTK7) (NM_152881) Human Over-expression Lysate (LS005754)
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No publications found for LS005754
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