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Product Info Summary
| SKU: | A02957 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CCK-4 PTK7 Monoclonal Antibody
SKU/Catalog Number
A02957
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CCK-4 PTK7 Monoclonal Antibody catalog # A02957. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CCK-4 PTK7 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02957)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
4F9
Isotype
IgG
Immunogen
Purified recombinant fragment of human CCK-4 expressed in E. Coli.
Reactive Species
A02957 is reactive to PTK7 in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
118.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02957 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis using CCK-4 Monoclonal Antibody against HeLa (1), A431 (2), HCT116 (3), Caco2 (4), HepG2 (5) and MCF-7 (6) cell lysate.
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Immunohistochemistry analysis of paraffin-embedded lung cancer tissues with DAB staining using CCK-4 Monoclonal Antibody.
Specific Publications For Anti-CCK-4 PTK7 Monoclonal Antibody (A02957)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-CCK-4 PTK7 Monoclonal Antibody
Question
We are currently using anti-CCK-4 Monoclonal antibody A02957 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
The anti-CCK-4 Monoclonal antibody (A02957) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-11
Question
I have attached the WB image, lot number and protocol we used for leukemic t-cell using anti-CCK-4 Monoclonal antibody A02957. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-31
Question
I was wanting to use your anti-CCK-4 Monoclonal antibody for IHC for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2019-05-02
Answer
You can see on the product datasheet, A02957 anti-CCK-4 Monoclonal antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-02
Question
Is a blocking peptide available for product anti-CCK-4 Monoclonal antibody (A02957)?
G. Mitchell
Verified customer
Asked: 2018-05-17
Answer
We do provide the blocking peptide for product anti-CCK-4 Monoclonal antibody (A02957). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-05-17
Question
Is this A02957 anti-CCK-4 Monoclonal antibody reactive to the isotypes of PTK7?
A. Lewis
Verified customer
Asked: 2017-06-07
Answer
The immunogen of A02957 anti-CCK-4 Monoclonal antibody is Purified recombinant fragment of human CCK-4 expressed in E. Coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-07
