CDC25A (Phospho-Ser178) Colorimetric Cell-Based ELISA Kit

CDC25A ELISA kit, phospho-specific, cell-based

The CDC25A (Phospho-Ser178) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CDC25A (Phospho-Ser178) protein expression profile in cells. The kit can be used for measuring the relative amounts of CDC25A (Phospho-Ser178) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CDC25A (Phospho-Ser178).

Product Info Summary

SKU: EKC2543
Size: 1 kit, containing two 96-well plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA

Product Name

CDC25A (Phospho-Ser178) Colorimetric Cell-Based ELISA Kit

See all CDC25A products

SKU/Catalog Number

EKC2543

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The CDC25A (Phospho-Ser178) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CDC25A (Phospho-Ser178) protein expression profile in cells. The kit can be used for measuring the relative amounts of CDC25A (Phospho-Ser178) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CDC25A (Phospho-Ser178).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

CDC25A (Phospho-Ser178) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2543)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC2543 is reactive to CDC25A in Human, Mouse, Rat samples

Application Guarantee

EKC2543 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-CDC25A Phospho-Ser178 Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-CDC25A Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For CDC25A (Source: Uniprot.Org, NCBI)

Gene Name

CDC25A

Full Name

M-phase inducer phosphatase 1

Weight

59087 MW

Superfamily

MPI phosphatase family

Alternative Names

M-phase inducer phosphatase 1;3.1.3.48;Dual specificity phosphatase Cdc25A;CDC25A; CDC25A CDC25A2 cell division cycle 25A M-phase inducer phosphatase 1|CDC25 homolog A|dual specificity phosphatase CDC25A

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CDC25A, check out the CDC25A Infographic

CDC25A infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CDC25A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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