CDC25A Antibodies

M-phase inducer phosphatase 1 (CDC25A)

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity.

Also dephosphorylates CDK2 in complex with cyclin E, in vitro. .

Gene Information

Human
Gene Name:	CDC25A
Uniprot:	P30304
Entrez:	993

Family

Belongs to:
MPI phosphatase family

Alternative Names

CDC25A; CDC25A2; CDC25A2-CAG isoform; cell division cycle 25 homolog A (S. cerevisiae); cell division cycle 25 homolog A (S. pombe); cell division cycle 25A; Dual specificity phosphatase Cdc25A; EC 3.1.3.48; M-phase inducer phosphatase 1

Molecular Weight

Mass (kDA):
59.087 kDA

Genomic Context

Human
Location:	3p21.31
Sequence:	3; NC_000003.12 (48157146..48188417, complement)

Diseases

• Malignant Neoplasms
• Neoplasms
• Carcinoma
• Cell Transformation, Neoplastic
• Retinoblastoma
• Malignant Neoplasm Of Breast
• Mammary Neoplasms
• Malignant Paraganglionic Neoplasm
• Leukemia
• Liver Carcinoma
• Malignant Neoplasm Of Lung
• Genomic Instability
• Genotoxic Stress

• Ataxia Telangiectasia
• Growth Arrest
• Ataxia
• Carcinogenesis
• Adenocarcinoma
• Malignant Squamous Cell Neoplasm
• Hyperplasia

Interacting Proteins

• RAF1
• YWHAB
• YWHAQ
• YWHAZ

Pathways

• Cell Cycle
• Cell Cycle Arrest
• Cell Proliferation
• S Phase
• Cell Growth
• Mitosis
• Cell Division
• Dna Replication
• Cell Death
• Dna Damage Checkpoint
• G1 Phase
• Dna Repair
• Cell Cycle Checkpoint

• Induction Of Apoptosis
• Proteolysis
• Rna Interference
• Oncogenesis
• M Phase
• Translation
• Localization

PTMs

• Phosphorylation
• Dephosphorylation
• Cleavage
• Acetylation
• Ubiquitination
• Methylation
• Reduction

• Demethylation
• Sulphation
• Phosphorothioation
• Hydroxylation
• Biotinylation
• Nitration
• Oxidation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CDC25A

Boster Bio: Best Uses Of The Forskolin CDC25A Marker Product Review

Boster Bio's Best Uses of Forskolin CDC25A Marker will be reviewing the history of Steven Boster as well the CDC25A marker. We'll also discuss the uses of Boster's products for special samples, applications and species as well as the latest advancements made in this marker.

CDC25A Cell Based ELISA Kit

CDC25A can be found in cancer cells as well as in inflammatory and cancer cells. It also identifies biomarkers. Boster Bio CDC25A Cell-Based ELISA Kit enables researchers to screen a wide range of activators and treatments by monitoring CDC25A protein levels in cells. It has a high sensitivity of up to picograms. This kit is compatible with both ELISA and immunohistochemistry.

This kit uses antibodies from the Boster Bio antibody library, which is comprised of over 16,000 different antibodies validated for use in ELISA, WB, and FC applications. Boster Bio provides primary antibodies free of charge, as well as monoclonal and polyclonal antibodies for rabbits and mice. Boster Bio offers a variety of other ELISA kits that are also highly sensitive and specific.

CDC25A - Marker

The CDC25A gene encodes a protein that is essential for centrosome stability. Cdc25A stability will be maintained by phosphorylation (S123), which prevents it amplifying following IR. This gene is also associated for centrosome amplification in S123AKI/KI-cells. CDC25A expression in cells can also be related to centrosome instability.

PP2A1 promotes the removal of CDC25A from cells by phosphorylating it. This enzyme is responsible in the isomerization and binding of CDC25C. This enzyme is involved both in the assembly and mitosis of microtubules. It can also occur in tumors or other cells.

CDC25A expression is increasing in a variety of human cancers. It is known to be a tumor suppressor, and may be useful for the diagnosis of liver cancer. Its expression is associated with low survival and poor prognosis. Cdc25A plays a role in the development liver cancer. However, more research is needed. This protein could be used to develop new treatments in the near future.

Primary antibodies with high affinity

The CDC25A marker for high affinity primary antibodies is a great tool to produce monospecific, highly-specific antigens. The CDC25A marker binds to a specific epitope in the fixed tissue. First, you must isolate the CDC25A marker in the tissue to generate high-affinity antibodies. The affinity constant of the specific antibody must then be determined.

CDC25A is a unique marker because it detects a subset of B cells. Without this marker, antibodies of high affinity cannot be produced by B cells. This resulted in the high affinity primary antibodies that can recognize and neutralize the autoantigen. These antibodies are the most effective type of primary antibody and are useful in many specialized applications, such as rheumatoid arthritis, autoimmune diseases, and tumors.

Additional markers, such as the CDC25A mark, can be used to determine antibody affinity. One way to determine the affinity of an antibody is by measuring its KD value. The KD value is proportional the antibody concentration. Therefore, the lower KD value indicates higher affinity. The University of California Davis was the first to recognize the CDC25A antibody marker.

The CDC25A marker is a good way to detect the Cdc25A specific antibody. It can be used for the detection of CDC25A-specific IgM/IgG-specific antibodies in mice. Many applications make use of the CDC25A mark. It is useful in identifying specific antigens and to identify new antigens. Researchers can identify new monoclonal antibodies by measuring Cdc25A.

The antigen in mouse serum is determined using a similar marker. The antibody is made to diacetylspermine, which is a monoamine with a mass of 200-300. These molecules share similar structures. GANP(r) mice produced anti-diacetylspermine antibodies, which were 5 times more specific than those from BALB/c mice.

History of Steven Boster

You can read his personal history to learn more about Steven Boster's life. Boster loved southern gospel music as a kid. He sang in the lower register to his family and friends. Boster loved sports, especially auto racing. He was always the first to help when his car stopped working at 2 a.m. He showed up for appointments even in below-freezing temperatures.

Steve Boster was born in Joliet (IL) and died on June 6, 2022 in Madison, WI after a battle against COVID-19. He is survived by his son Jonathan and daughter Crystal Boster. Also, three grandchildren, Natosha Pueck and Cory Boster. Steven was also survived his four siblings, Jack Boster and David Boster; sisters Kimberly Blanton (and Tammy) and nieces/nieces.