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Product Info Summary
| SKU: | LS170482 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of C-type lectin domain family 4, member C (CLEC4C), transcript variant 2 |
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Product info
Product Name
CLEC4C (NM_203503) Human Over-expression Lysate
See all DLEC/CLEC4C/BDCA-2 products
SKU/Catalog Number
LS170482
Size
100 µg
Description
Transient overexpression lysate of C-type lectin domain family 4, member C (CLEC4C), transcript variant 2
Storage & Handling
Cite This Product
CLEC4C (NM_203503) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS170482)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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CLEC4C Human Over-expression Lysates NM_203503.
Western blot validation of overexpression lysate (LS170482) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CLEC4C (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for CLEC4C (Source: Uniprot.org, NCBI)
Gene Name
CLEC4C
Full Name
C-type lectin domain family 4 member C
Weight
25.038kDa
Alternative Names
BDCA2; BDCA-2; BDCA2MGC125791; blood dendritic cell antigen 2 protein; Blood dendritic cell antigen 2; CD303 antigen; CD303; CLEC4C; CLECSF11; CLECSF11PRO34150; CLECSF7; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 11; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 7; C-type lectin domain family 4 member C; C-type lectin superfamily member 7; dendritic cell lectin b; Dendritic lectin; DLEC; DLECMGC125792; HECL; HECLMGC125793; MGC125789; PRO34150 CLEC4C BDCA-2, BDCA2, CD303, CLECSF11, CLECSF7, DLEC, HECL, PRO34150 C-type lectin domain family 4 member C C-type lectin domain family 4 member C|C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 11|C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 7|C-type lectin superfamily member 7|blood dendritic cell antigen 2 protein|dendritic cell lectin b|dendritic lectin
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CLEC4C, check out the CLEC4C Infographic
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In this infographic, you will see the following information for CLEC4C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For CLEC4C (NM_203503) Human Over-expression Lysate (LS170482)
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