Product Info Summary
SKU: | LS004253 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of CTAGE family, member 5 (CTAGE5), transcript variant 2 |
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Product info
Product Name
CTAGE5 (MIA2) (NM_203354) Human Over-expression Lysate
SKU/Catalog Number
LS004253
Size
100 µg
Description
Transient overexpression lysate of CTAGE family, member 5 (CTAGE5), transcript variant 2
Storage & Handling
Cite This Product
CTAGE5 (MIA2) (NM_203354) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS004253)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
Click image to see more details
CTAGE5 (MIA2) Human Over-expression Lysates NM_203354.
Western blot validation of overexpression lysate (LS004253) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CTAGE5 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for MIA2 (Source: Uniprot.org, NCBI)
Gene Name
MIA2
Full Name
Melanoma inhibitory activity protein 2
Weight
159.836kDa
Superfamily
MIA/OTOR family
Alternative Names
FLJ22404; melanoma inhibitory activity 2; melanoma inhibitory activity protein 2 MIA2 CTAGE5, MEA6, MGEA, MGEA11, MGEA6, TALI MIA SH3 domain ER export factor 2 melanoma inhibitory activity protein 2|CTAGE family member 5, ER export factor|CTAGE family, member 5|MIA protein 2|TANGO1-like|cutaneous T-cell lymphoma-associated antigen 5|melanoma inhibitory activity 2|meningioma expressed antigen 6 (coiled-coil proline-rich)|meningioma-expressed antigen 6/11|protein cTAGE-5
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on MIA2, check out the MIA2 Infographic
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In this infographic, you will see the following information for MIA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For CTAGE5 (MIA2) (NM_203354) Human Over-expression Lysate (LS004253)
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