Product Info Summary
SKU: | LS054431 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of DnaJ (Hsp40) homolog, subfamily C, member 10 (DNAJC10) |
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Product info
Product Name
DNAJC10 (NM_018981) Human Over-expression Lysate
SKU/Catalog Number
LS054431
Size
100 µg
Description
Transient overexpression lysate of DnaJ (Hsp40) homolog, subfamily C, member 10 (DNAJC10)
Storage & Handling
Cite This Product
DNAJC10 (NM_018981) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS054431)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Protein Target Info & Infographic
Gene/Protein Information for DNAJC10 (Source: Uniprot.org, NCBI)
Gene Name
DNAJC10
Full Name
DnaJ homolog subfamily C member 10
Weight
91.08kDa
Alternative Names
DKFZp434J1813; DnaJ (Hsp40) homolog, subfamily C, member 10; dnaJ homolog subfamily C member 10; ERdj5; ER-resident protein ERdj5; J-domain-containing protein disulfide isomerase-like protein; JPDI; macrothioredoxin; MGC104194; MTHr DNAJC10 ERdj5, JPDI, MTHr, PDIA19 DnaJ heat shock protein family (Hsp40) member C10 dnaJ homolog subfamily C member 10|DnaJ (Hsp40) homolog, subfamily C, member 10|ER-resident protein ERdj5|J-domain-containing protein disulfide isomerase-like protein|endoplasmic reticulum DNA J domain-containing protein 5|macrothioredoxin|protein disulfide isomerase family A, member 19
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on DNAJC10, check out the DNAJC10 Infographic
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In this infographic, you will see the following information for DNAJC10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For DNAJC10 (NM_018981) Human Over-expression Lysate (LS054431)
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No publications found for LS054431
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