Product Info Summary
SKU: | LS025897 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of ring finger protein 19A (RNF19A), transcript variant 1 |
Customers Who Bought This Also Bought
Product info
Product Name
DORFIN (RNF19A) (NM_183419) Human Over-expression Lysate
SKU/Catalog Number
LS025897
Size
100 µg
Description
Transient overexpression lysate of ring finger protein 19A (RNF19A), transcript variant 1
Storage & Handling
Cite This Product
DORFIN (RNF19A) (NM_183419) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS025897)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
Click image to see more details
DORFIN (RNF19A) Human Over-expression Lysates NM_015435.
Western blot validation of overexpression lysate (LS025897) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RNF19A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
DORFIN (RNF19A) Human Over-expression Lysates NM_183419.
Western blot validation of overexpression lysate (LS025897) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RNF19A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for RNF19A (Source: Uniprot.org, NCBI)
Gene Name
RNF19A
Full Name
E3 ubiquitin-protein ligase RNF19A
Weight
90.696kDa
Superfamily
RBR family
Alternative Names
DKFZp566B1346; dorfin; Double ring-finger protein; E3 ubiquitin-protein ligase RNF19A; EC 6.3.2; EC 6.3.2.-; p38; protein p38 interacting with transcription factor Sp1; RING finger protein 19 isoform; ring finger protein 19; ring finger protein 19Ap38 protein; ring-IBR-ring domain containing protein Dorfin; RNF19 RNF19A RNF19 ring finger protein 19A, RBR E3 ubiquitin protein ligase E3 ubiquitin-protein ligase RNF19A|double ring-finger protein|protein p38 interacting with transcription factor Sp1|ring finger protein 19A, E3 ubiquitin protein ligase|ring-IBR-ring domain containing protein Dorfin
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on RNF19A, check out the RNF19A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RNF19A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For DORFIN (RNF19A) (NM_183419) Human Over-expression Lysate (LS025897)
Hello CJ!
No publications found for LS025897
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful:
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used DORFIN (RNF19A) (NM_183419) Human Over-expression Lysate?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For DORFIN (RNF19A) (NM_183419) Human Over-expression Lysate
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question