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Product Info Summary
| SKU: | LS009775 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of eukaryotic translation initiation factor 4A, isoform 3 (EIF4A3) |
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Product info
Product Name
EIF4A3 (NM_014740) Human Over-expression Lysate
SKU/Catalog Number
LS009775
Size
100 µg
Description
Transient overexpression lysate of eukaryotic translation initiation factor 4A, isoform 3 (EIF4A3)
Storage & Handling
Cite This Product
EIF4A3 (NM_014740) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS009775)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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EIF4A3 Human Over-expression Lysates NM_014740.
Western blot validation of overexpression lysate (LS009775) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with EIF4A3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for EIF4A3 (Source: Uniprot.org, NCBI)
Gene Name
EIF4A3
Full Name
Eukaryotic initiation factor 4A-III
Weight
46.871kDa
Superfamily
DEAD box helicase family
Alternative Names
ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DDX48; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; DEAD box protein 48; DKFZp686O16189; EC 3.6.1; EC 3.6.4.13; EIF4AIII; eIF-4A-III; eIF4A-III; eukaryotic initiation factor 4A-III; Eukaryotic initiation factor 4A-like NUK-34; Eukaryotic translation initiation factor 4A isoform 3; eukaryotic translation initiation factor 4A; eukaryotic translation initiation factor 4A3; hNMP 265; KIAA0111eukaryotic translation initiation factor 4A, isoform 3; MGC10862; NMP 265; NMP265; Nuclear matrix protein 265; NUK34 EIF4A3 DDX48, Fal1, MUK34, NMP265, NUK34, RCPS, eIF-4A-III, eIF4A-III, eIF4AIII eukaryotic translation initiation factor 4A3 eukaryotic initiation factor 4A-III|ATP-dependent RNA helicase DDX48|ATP-dependent RNA helicase eIF4A-3|DEAD (Asp-Glu-Ala-Asp) box polypeptide 48|DEAD box protein 48|NMP 265|eukaryotic initiation factor 4A-like NUK-34|nuclear matrix protein 265
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on EIF4A3, check out the EIF4A3 Infographic
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In this infographic, you will see the following information for EIF4A3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For EIF4A3 (NM_014740) Human Over-expression Lysate (LS009775)
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