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- Table of Contents
ELISA Kits
Boster ELISA kits offer a comprehensive selection for cytokines, growth factors, hormones, chemokines, and other biomarkers across various species including human, mouse, and rat. Each kit is meticulously developed, validated, and quality-controlled in-house to ensure top performance.
Why Choose Boster ELISA Kits
Comprehensive, Verified Coverage
Over 1,200 targets across human, mouse, rat, and other species — including cytokines, hormones, growth factors, chemokines, and biomarkers. Boster’s ELISA kits are meticulously developed, validated, and quality-controlled in-house, ensuring traceable performance data beyond mere catalog claims.
This enables you to standardize assays seamlessly across projects and teams, backed by consistent documentation and robust QC practices for reliable results every time.
At a glance
1,200+
Targets validated in-house
Multi-species
Human • Mouse • Rat • More
- In-house workflow: development → validation → QC
- Traceable data: performance metrics documented
- Not aggregated: avoids catalog-only claims
Comprehensive Validation and Quality Assurance
Each ELISA kit batch is evaluated in-house for specificity, linearity, recovery, precision, stability, and lot-to-lot consistency. Precision targets are defined by repeatability and between-lot consistency to support reproducible, publication-grade results.
- Reproducibility: Rigorous multi-lot testing to minimize variance
- Precision: Repeatability (Intra-assay CV ≤ 10%) and lot-to-lot consistency (CV ≤ 15%)
- Linearity: Comprehensive dilution series testing across the dynamic range for accurate quantitation
- Specificity: Cross-reactivity assessments against related proteins
- Stability: Long-term performance tracking to verify consistent kit performance
- Recovery: Spike-and-recovery protocols in relevant matrices to validate accuracy
Typical acceptance targets
Linearity
80–120%
Recovery
80–120%
Precision
Repeatability (Intra-assay CV) ≤ 10%
Lot-to-lot consistency (CV) ≤ 15%
Lot-to-lot consistency (CV) ≤ 15%
Lot-to-lot consistency is continuously monitored to support reproducible results across projects.
Five ELISA Formats for Every Research Need
A complete selection guide — choose the right ELISA format by speed, sensitivity, flexibility, or multiplex needs.
Explore by format
Compare at a glance
| Format | Assay time | Flexibility | Best for | Trade-offs |
|---|---|---|---|---|
| PicoKine® ELISA Kits | Standard ELISA workflow | Low–Medium | Classic, stable, reliable, and widely used | Less customizable than EZ-Set |
| Quick ELISA (90 min) | ~90 minutes | Low | Time-sensitive screening | Not the most tunable format |
| High Sensitivity ELISA | Standard-to-extended | Low–Medium | Low abundance / complex matrices | Technique consistency matters more |
| EZ-Set Antibody Pairs | User-defined | High | DIY assay building & optimization | Requires development effort |
| Multiplex ELISA | Panel workflow | Medium | Panels with limited sample | May require specific readout setup |
Pick your priority
What to do next
Need validation details?
See how we evaluate linearity, recovery, precision, specificity, stability, and lot-to-lot consistency.
View QC & Validation →
Want the best price now?
Limited-time offer on top best-selling ELISA kits.
View 40% OFF promotion →
If you are unsure about sample type, matrix effects, or expected concentration range, contact technical support before ordering.
Format Details
Compare ELISA formats with an on-page guide. Expand a format to see best-fit scenarios, what you get, and trade-offs.
PicoKine® ELISA Kits: When should I choose a ready-to-run sandwich ELISA?
Best for: Achieving publication-grade quantitation with a dependable, ready-to-use sandwich ELISA.
Why choose it: Benefit from a standardized workflow that ensures reproducible results across various experiments and batch lots.
What you get: Receive a comprehensive kit configuration with documentation that meets rigorous quality control standards.
Trade-offs: Offers less flexibility compared to EZ-Set and generally requires more time than Quick formats.
Quick ELISA (90 min): When should I prioritize speed?
Best for: Rapid high-throughput screening and urgent experiments demanding quick results.
Why choose it: Efficient workflow tailored for delivering results in approximately 90 minutes, enhancing productivity.
What you get: Accelerated assay procedures with comprehensive, step-by-step instructions for ease of use.
Trade-offs: Limited customization options; opt for High Sensitivity formats for ultra-low detection needs.
High Sensitivity ELISA: When do I need ultra-low-abundance detection?
Best for: Ultra-low-abundance targets, limited sample volume, or complex matrices.
Why choose it: Optimized to improve detection in the low concentration range.
What you get: A comprehensive high-sensitivity kit setup with expert guidance, ensuring alignment with stringent quality control standards.
Trade-offs: Consistent technique is crucial; careful sample preparation and precise dilution strategies are essential.
EZ-Set Antibody Pairs: When should I build or customize my own ELISA?
Best for: Experienced ELISA users seeking flexibility, customization, or exploratory assay development.
Why choose it: Offers core assay “building blocks” allowing you to customize plates, incubation, and workflow.
What you get: Matched antibody pair and standards/core reagents tailored to your EZ-Set configuration.
Trade-offs: Involves development and optimization efforts, but provides maximum control.
Multiplex ELISA: When do I need panels or pathway profiling?
Best for: Simultaneously measuring multiple biomarkers in a single sample, especially useful for cytokine panels and pathway-related proteins.
Why choose it: Enhances efficiency and data richness when sample volume is limited, yet multiple target analysis is necessary.
What you get: A comprehensive panel-based workflow along with expert guidance on expected readout results.
Trade-offs: Increased workflow complexity; ensure confirmation of readout setup requirements prior to ordering.
ELISA QC & Validation Standards
QC at a glance — see how each batch is verified before release.
Every ELISA kit lot is validated in-house against core performance checks. Below are visual QC snapshots that show what we verify and how results are evaluated — with representative examples. Full criteria and methods are documented on the QC page.
Example kit (specificity): Human Cathepsin D ELISA Kit PicoKine® (EK0672)
Example kit (sensitivity): Human IL-6/Interleukin-6 ELISA Kit PicoKine® (EK0410)
Example kit (intra/inter consistency): Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine® (EK0527)
Example kit (linearity): Human EGF ELISA Kit PicoKine® (EK0325)
Example kit (recovery): Human TFF1/PS2 ELISA Kit PicoKine® (EK1232)
Example kit (reproducibility): Human MPO/Myeloperoxidase ELISA Kit PicoKine® (EK0850)
Example kit (stability): Human EGF ELISA Kit PicoKine® (EK0325)
ELISA Testing Service
Send your samples — we run the ELISA and deliver publication-ready results.
Need reliable ELISA data but short on time, staff, or bandwidth? Boster’s ELISA testing service helps you move faster with a structured workflow, clear documentation, and results you can confidently interpret and report.
- Run by ELISA specialists: backed by an ELISA manufacturer’s expertise
- Robust & reproducible: consistent execution, controls, and reporting
- Flexible options: multiplex ELISA and custom ELISA development available
Publications Using Boster ELISA Kits
Six selected peer-reviewed publications citing Boster ELISA kits.
Glioblastoma remodelling of human neural circuits decreases survival
NATURE | May 03, 2023 | IF 64.8
Boster Kit & SKU: Human THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine® (EK0899)
Article / DOI: Publisher page | 10.1038/s41586-023-06036-1
Astrocytic interleukin-3 programs microglia and limits Alzheimer’s disease
NATURE | Jul 14, 2021 | IF 49.96
Boster Kit & SKU: Human IL-3 ELISA Kit PicoKine® (EK0402)
Article / DOI: Publisher page | 10.1038/s41586-021-03734-6
Sleep modulates haematopoiesis and protects against atherosclerosis
NATURE | Feb 13, 2019 | IF 43.07
Boster Kit & SKU: Mouse HCRT/Orexin A ELISA Kit PicoKine®(EK0445)
Article / DOI: Publisher page | 10.1038/s41586-019-0948-2
Inflammatory and anti-inflammatory cytokines balance to drive intraarterial thrombus resolution
CELL | Apr 07, 2025 | IF 42.5
Boster Kit & SKU: Mouse IL-17A ELISA Kit PicoKine®(EK0790)
Article / DOI: Publisher page | 10.1016/j.cell.2025.03.005
Neutrophil membrane-coated nanoparticles inhibit synovial inflammation and alleviate joint damage in inflammatory arthritis
Nature Nanotechnology | Sep 03, 2018 | IF 37.49
Boster Kit & SKU: Mouse MMP-3 ELISA Kit PicoKine®(EK0461)
Article / DOI: Publisher page | 10.1038/s41565-018-0254-4
Decline in IGF1 in the bone marrow microenvironment initiates hematopoietic stem cell aging
Cell Stem Cell | Aug 05, 2021 | IF 24.63
Boster Kit & SKU: Mouse IGF-2 ELISA Kit PicoKine® (EK0381)
Article / DOI: Publisher page | 10.1016/j.stem.2021.03.017
Testimonials
What researchers say about Boster ELISA kits.
I used the ELISA kit to detect the BDNF level in several samples, including brain tissues (cortex and hippocampus), cultured astrocyte (cell lysis) and astrocyte conditioned medium. The kit works very well for cell lysis and brain tissues according to the instruction. For the cell medium, I suggest that users need modify the protocol a little bit, such as prolong the incubation time for samples in the wells for overnight. The kit has a good quality.
Kit & SKU: Mouse BDNF ELISA Kit PicoKine® (EK0309)
View Product →
JUNHUI WANG
Job Position: Research Associate
University/Organization: University of Toronto
The instructions are clear, concise, and the process is more streamlined than other kits. I highly recommend investing in these kits compared to other, less expensive ones.
Kit & SKU: Human MMP-9 ELISA Kit PicoKine® (EK0465)
View Product →
Irina Molina
Job Position: Graduate student
University/Organization:
University of Louisville
Microbiology & immunology
I used the kit to test the ADAMTS1 protein level in human endothelial cell lysates. The instructions are clear and the kit worked the first time with reproducible results. I will recommend this product to my colleagues.
Kit & SKU: Human ADAMTS1 ELISA Kit PicoKine® (EK1340)
View Product →
Huiyuan Tang
Job Position: Lab Specialist
University/Organization: University of Utah
I am very much satisfied with the product Human IL-1 Beta PicoKine™ ELISA Kit (EK0392) purchased from Boster Bio. I used the Boster Bio ElISA kit for the first time and I am very happy with this product. After trying the IL-1beta kit, I have purchased two other cytokine detection ELISA kits from Boster Bio. The methodology of Human IL-1 Beta PicoKine™ ELISA Kit is simplified and user friendly. It required minimum optimization for my experiments. The written instructions provided with the kit is very useful for running the ELISA. The results obtained so far have been consistent and reproducible. For my future ELISA experiments, I'll be buying the ELISA kit from Boster Bio.
Kit & SKU: Human IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine® (EK0392)
View Product →
Dhruva Bhattacharya
Job Position: Postdoctoral Research Associate
University/Organization: University of Arizona
Terms & Conditions
- • Excludes bulk and custom orders
- • Valid on Top 100 ELISA kits only
- • 40% discount is off list price
- • Shipping charges apply
- • Not applicable to previous purchases
- • Available to direct customers in USA only
- • Cannot be combined with other offers
- • May be discontinued at any time
- • Void where prohibited by company or institutional policy
Top 100 ELISA Kits — Now 40% Off
Stretch your funding without compromising your data. Save 40% on our Top 100 ELISA Kits—selected for high citation impact, proven reproducibility, and validated performance.
Why this offer matters
- • 40% off list price on eligible Top 100 ELISA Kits
- • Chosen for strong scientific impact and reputation in published research
- • Built to support trusted, publishable data
What you’re getting
- • Pre-coated 96-well ELISA kits
- • Comparable to R&D Systems’ Quantikine® kits (not the DuoSet® type)
- • Rigorous validation for specificity, sensitivity, and batch-to-batch consistency
ELISA Resource Center
Learn the ELISA fundamentals, follow step-by-step protocols, troubleshoot common issues, and analyze your data with confidence.
Start here: a simple ELISA workflow
Visit ELISA Technical Resource Center
Browse technical blogs, how-tos, optimization tips, and ELISA troubleshooting resources in one place.
FAQs
Questions about data reliability, validation, and Boster’s quality guarantee for ELISA kits.
Q1. How do I know Boster ELISA kits deliver real, reproducible data?
Boster ELISA kits are meticulously developed, validated, and quality-controlled in-house. Each batch undergoes rigorous testing for critical performance metrics, including specificity, linearity, recovery, precision, stability, and lot-to-lot consistency. This ensures that our kits deliver reliable and traceable performance, perfectly aligned with datasheet specifications.
Q2. What does the Boster Quality Guarantee cover for ELISA kits?
We assure that our ELISA kits adhere to the exact specifications detailed on our datasheets and are rigorously tested before shipping. Should a kit not perform as expected for its specified application or reactivity, we commit to a thorough investigation and will provide a refund or free replacement in accordance with our guarantee terms. Your satisfaction and trust in our products are our top priorities.
Q3. What happens if I run into problems during my ELISA experiment?
If you encounter an issue, our technical support team will respond with personalized troubleshooting advice within 24 hours. We’ll help you identify likely root causes (e.g., sample handling, incubation/wash conditions, matrix effects) and recommend corrective actions.
Q4. If support can’t make my experiment work, what are my options?
If we can’t resolve the problem and make your experiment work, we will provide a full refund or a free replacement, in line with the Boster Quality Guarantee. Full terms and conditions are provided in the guarantee section below.
Support
Need help or have questions?
Email us and we’ll route your message to the right team. Response times and detailed terms are listed in the guarantee section below.
- Sales: sales@bosterbio.com
- Orders: orders@bosterbio.com
- Customer/Technical Support: support@bosterbio.com
- Business Development / Escalations: boster@bosterbio.com