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Product Info Summary
| SKU: | EKC2070 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
FGFR1 (Phospho-Tyr654) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2070
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The FGFR1 (Phospho-Tyr654) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FGFR1 (Phospho-Tyr654) protein expression profile in cells. The kit can be used for measuring the relative amounts of FGFR1 (Phospho-Tyr654) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on FGFR1 (Phospho-Tyr654).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
FGFR1 (Phospho-Tyr654) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2070)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2070 is reactive to FGFR1 in Human, Mouse, Rat samples
Validated Sample Types
Cell lines
Application Guarantee
EKC2070 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-FGFR1 (Phospho-specific) Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-FGFR1 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
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Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from 293 cells treated with Insulin 0.01U/ml 15'
Protein Target Info & Infographic
Gene/Protein Information For FGFR1 (Source: Uniprot.Org, NCBI)
Gene Name
FGFR1
Full Name
Fibroblast growth factor receptor 1
Weight
91.868kDa
Superfamily
protein kinase superfamily
Alternative Names
basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; CD331 antigen; CD331; CEK; EC 2.7.10; EC 2.7.10.1; FGF R1; FGFBR; FGFR1; FGFR-1; fibroblast growth factor receptor 1; FLGH3; FLJ99988; Flt-2; FLT2H4; Fms-like tyrosine kinase 2; fms-related tyrosine kinase 2; H2; H5; HBGFR; heparin-binding growth factor receptor; hydroxyaryl-protein kinase; KAL 2; KAL2; N-SAM; OGD; Proto-oncogene c-Fgr; soluble FGFR1 variant 1; soluble FGFR1 variant 2 FGFR1 BFGFR, CD331, CEK, ECCL, FGFBR, FGFR-1, FLG, FLT-2, FLT2, HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD, bFGF-R-1 fibroblast growth factor receptor 1 fibroblast growth factor receptor 1|FGFR1/PLAG1 fusion|FMS-like tyrosine kinase 2|basic fibroblast growth factor receptor 1|fms-related tyrosine kinase 2|heparin-binding growth factor receptor|hydroxyaryl-protein kinase|proto-oncogene c-Fgr
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FGFR1, check out the FGFR1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FGFR1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For FGFR1 (Phospho-Tyr654) Colorimetric Cell-Based ELISA Kit (EKC2070)
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Customer Q&As
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3 Customer Q&As for FGFR1 (Phospho-Tyr654) Colorimetric Cell-Based ELISA Kit
Question
Does EKC2070 contain recombinant antibodies?
Verified customer
Asked: 2020-11-18
Answer
The FGFR1 (Phospho-Tyr654) Colorimetric Cell-Based ELISA Kit (EKC2070) does not contain recombinant antibodies.
Boster Scientific Support
Answered: 2020-11-20
Question
What is the immunized animal host for HRP-Conjugated Anti-Rabbit IgG Antibody and HRP-Conjugated Anti-Mouse IgG Antibody?
Verified customer
Asked: 2020-11-18
Answer
For the FGFR1 (Phospho-Tyr654) Colorimetric Cell-Based ELISA Kit (EKC2070), the immunized animal host is Goat for both antibodies.
Boster Scientific Support
Answered: 2020-11-20
Question
Does EKC2070 contain any recombinant protein?
Verified customer
Asked: 2020-11-18
Answer
The FGFR1 (Phospho-Tyr654) Colorimetric Cell-Based ELISA Kit (EKC2070) kit does not contain recombinant proteins.
Boster Scientific Support
Answered: 2020-11-20
