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Product Info Summary
| SKU: | A00098-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-FGFR1 Antibody (Center)
SKU/Catalog Number
A00098-2
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-FGFR1 Antibody (Center) (Catalog # A00098-2). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-FGFR1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00098-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This FGFR1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 291-324 amino acids from the Central region of human FGFR1.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00098-2 is reactive to FGFR1 in Human, Mouse
Calculated molecular weight
91.9 kDa
Background of FGFR1
Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00098-2 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
FC: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysates from Hela, HepG2, MCF-7, SH-SY5Y, mouse NIH/3T3 cell line (from left to right), using FGFR1 Antibody (Center). A00098-2 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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Flow cytometric analysis of SH-SY5Y cells using FGFR1 Antibody (Center)(green) compared to an isotype control of rabbit IgG(blue). A00098-2 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Specific Publications For Anti-FGFR1 Antibody (Center) (A00098-2)
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