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Product Info Summary
| SKU: | LS002290 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of forkhead box G1 (FOXG1) |
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Product info
Product Name
FOXG1 (NM_005249) Human Over-expression Lysate
SKU/Catalog Number
LS002290
Size
100 µg
Description
Transient overexpression lysate of forkhead box G1 (FOXG1)
Storage & Handling
Cite This Product
FOXG1 (NM_005249) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS002290)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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FOXG1 Human Over-expression Lysates NM_005249.
Western blot validation of overexpression lysate (LS002290) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with FOXG1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for FOXG1 (Source: Uniprot.org, NCBI)
Gene Name
FOXG1
Full Name
Forkhead box protein G1
Weight
52.352kDa
Alternative Names
BF-1; BF1HBF-2; BF2; BF-2; Brain factor 1; Brain factor 2; FHKL3; FKHL1forkhead-like 1; FKHL2; FKHL3; FKHL4; forkhead box G1; forkhead box G1A; forkhead box G1B; forkhead box G1C; Forkhead box protein G1A; Forkhead box protein G1B; Forkhead box protein G1C; forkhead-like 3; forkhead-like 4; Forkhead-related protein FKHL1; Forkhead-related protein FKHL2; Forkhead-related protein FKHL3; FOXG1A; FOXG1Bforkhead-like 2; FOXG1C; HBF-1; hBF-2; HBF-3; HFK1HBF-G2; HFK2HBF2; HFK3KHL2; oncogene QIN; QINFKH2forkhead box protein G1 FOXG1 BF1, BF2, FHKL3, FKH2, FKHL1, FKHL2, FKHL3, FKHL4A, FOXG1B, FOXG1C, HBF-1, HBF-2, HBF-3, HBF-G2, HBF2, HFK1, HFK2, HFK3, KHL2, QIN, FOXG1 forkhead box G1 forkhead box protein G1|brain factor 1|brain factor 2|forkhead-like 1|forkhead-like 2|forkhead-like 3|forkhead-like 4|oncogene QIN
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on FOXG1, check out the FOXG1 Infographic
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In this infographic, you will see the following information for FOXG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For FOXG1 (NM_005249) Human Over-expression Lysate (LS002290)
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