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Product Info Summary
| SKU: | A01852-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-FOXG1 Antibody (Center)
SKU/Catalog Number
A01852-1
Size
80 µl
Description
Boster Bio Anti-FOXG1 Antibody (Center) (Catalog # A01852-1). Tested in WB, IF, Flow Cytometry application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-FOXG1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01852-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This FOXG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-252 amino acids from the Central region of human FOXG1.
Reactive Species
A01852-1 is reactive to FOXG1 in Human, Mouse
Calculated molecular weight
52.4 kDa
Background of FOXG1
This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of the brain and telencephalon.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01852-1 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IF: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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Fluorescent confocal image of A549 cell stained with FOXG1 Antibody (Center). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0. 1%, 10 min), then incubated with FOXG1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). FOXG1 immunoreactivity is localized to Nucleus significantly.
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Anti-FOXG1 Antibody (Center) at 1:1000 dilution + human brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 52 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of FOXG1 Antibody (Center) (Cat. #A01852-1) in mouse brain tissue lysates (35ug/lane). FOXG1 (arrow) was detected using the purified Pab.
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FOXG1 Antibody (Center) (Cat. #A01852-1) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-FOXG1 Antibody (Center) (A01852-1)
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