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Product Info Summary
| SKU: | LS010772 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of splicing factor, arginine/serine-rich 13A (SFRS13A), transcript variant 1 |
Product info
Product Name
FUSIP1 (SRSF10) (NM_006625) Human Over-expression Lysate
SKU/Catalog Number
LS010772
Size
100 µg
Description
Transient overexpression lysate of splicing factor, arginine/serine-rich 13A (SFRS13A), transcript variant 1
Storage & Handling
Cite This Product
FUSIP1 (SRSF10) (NM_006625) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS010772)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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FUSIP1 (SRSF10) Human Over-expression Lysates NM_006625.
Western blot validation of overexpression lysate (LS010772) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SRSF10 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for SRSF10 (Source: Uniprot.org, NCBI)
Gene Name
SRSF10
Full Name
Serine/arginine-rich splicing factor 10
Weight
31.301kDa
Superfamily
splicing factor SR family
Alternative Names
40 kDa SR-repressor protein; FLJ43846; FUS interacting protein (serine-arginine rich) 1; FUS-interacting protein (serine-arginine rich) 2; FUS-interacting serine-arginine-rich protein 1; FUSIP1DKFZp686H0644; FUSIP2; neural-salient SR protein; NSSR; serine/arginine-rich splicing factor 10; serine-arginine repressor protein (40 kDa); SFRS13; SFRS13A; Splicing factor SRp38; splicing factor, arginine/serine-rich 13; Splicing factor, arginine/serine-rich 13ATLS-associated protein with Ser-Arg repeats; SR splicing factor 10; SRp38; SRrp40TLS-associated protein with SR repeats; TASR1TLS-associated SR SRSF10 FUSIP1, FUSIP2, NSSR, PPP1R149, SFRS13, SFRS13A, SRp38, SRrp40, TASR, TASR1, TASR2 serine and arginine rich splicing factor 10 serine/arginine-rich splicing factor 10|40 kDa SR-repressor protein|FUS interacting protein (serine-arginine rich) 1|FUS-interacting protein (serine-arginine rich) 2|SR splicing factor 10|TLS-associated SR protein|TLS-associated protein TASR|TLS-associated protein with SR repeats|TLS-associated protein with Ser-Arg repeats|TLS-associated serine-arginine protein 1|TLS-associated serine-arginine protein 2|neural-salient SR protein|protein phosphatase 1, regulatory subunit 149|serine-arginine repressor protein (40 kDa)|splicing factor SRp38|splicing factor, arginine/serine-rich 13|splicing factor, arginine/serine-rich 13A
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on SRSF10, check out the SRSF10 Infographic
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In this infographic, you will see the following information for SRSF10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For FUSIP1 (SRSF10) (NM_006625) Human Over-expression Lysate (LS010772)
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