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Product Info Summary
| SKU: | LS128674 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of prokineticin receptor 2 (PROKR2) |
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Product info
Product Name
GPR73B (PROKR2) (NM_144773) Human Over-expression Lysate
See all Prokineticin R2/PROKR2 products
SKU/Catalog Number
LS128674
Size
100 µg
Description
Transient overexpression lysate of prokineticin receptor 2 (PROKR2)
Storage & Handling
Cite This Product
GPR73B (PROKR2) (NM_144773) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS128674)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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GPR73B (PROKR2) Human Over-expression Lysates NM_144773.
Western blot validation of overexpression lysate (LS128674) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PROKR2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PROKR2 (Source: Uniprot.org, NCBI)
Gene Name
PROKR2
Full Name
Prokineticin receptor 2
Weight
43.996kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
dJ680N4.3; G protein-coupled receptor 73-like 1; GPR73bG-protein coupled receptor 73-like 1; GPR73L1; GPR73L1Kallmann syndrome 3 (autosomal dominant); GPRg2; GPRg2KAL3; KAL3; PKR2; PKR2PK-R2; Prokineticin R2; prokineticin receptor 2; ProkineticinR2; PROKR2 PROKR2 GPR73L1, GPR73b, GPRg2, HH3, KAL3, PKR2, dJ680N4.3 prokineticin receptor 2 prokineticin receptor 2|G protein-coupled receptor 73-like 1|G-protein coupled receptor I5E|PK-R2
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PROKR2, check out the PROKR2 Infographic
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In this infographic, you will see the following information for PROKR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For GPR73B (PROKR2) (NM_144773) Human Over-expression Lysate (LS128674)
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No publications found for LS128674
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