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Product Info Summary
| SKU: | EK1161 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Azurocidin ELISA Kit PicoKine®
View all Azurocidin/CAP37/HBP ELISA kits
SKU/Catalog Number
EK1161
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Azurocidin ELISA Kit PicoKine™ (96 Tests). Quantitate Human AZU1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Azurocidin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1161)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: I27-P248
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1161 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1161 is reactive to AZU1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1161 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Azurocidin/CAP37/HBP
Azurocidin, also known as cationic antimicrobial protein CAP37 or heparin-binding protein (HBP), is a protein that in humans is encoded by the AZU1 gene. This encoded protein is a member of the serine protease gene family, but it is not a serine proteinase, because the active site serine and histidine residues are replaced. Azurocidin is mapped to 19p13.3. The protein encoded by this gene is an azurophil granule antibiotic protein, with antibacterial activity. It is also an important multifunctional inflammatory mediator. In addition to it, Azurocidin is also a specific chemoattractant for monocytes. It lacks the chemotactic activity for neutrophils and lymphocytes, and this gene is probably responsible for the wave of monocytes that follows the initial wave of PMNs typical of the inflammatory response.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1161-CAP | Anti-Human AZU1 Pre-coated 96-well strip microplate | 1 |
| EK1161-ST | Human AZU1 Standard | 2 vials, 10 ng/tube |
| EK1161-DA | Human AZU1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.021 | 0.063 | 0.133 | 0.200 | 0.334 | 0.505 | 1.059 | 1.887 |
Data Example Images
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Human Azurocidin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Azurocidin/CAP37/HBP, Dilution ratio of 1:20, concentration in serum is around 10 ng/ml..
Some articles we found to cite concentrations of Azurocidin/CAP37/HBP in samples: 23808364 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Azurocidin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 178 | 606 | 1031 | 175 | 567 | 1093 |
| Standard deviation | 9.79 | 39.99 | 75.56 | 9.62 | 38.55 | 96.18 |
| CV (%) | 5.5% | 6.6% | 7.3% | 5.5% | 6.8% | 8.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Azurocidin/CAP37/HBP in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 178 | 162 | 182 | 159 | 170 | 9.9 | 5.8% |
| Sample 2 | 606 | 547 | 628 | 536 | 579 | 38.73 | 6.6% |
| Sample 3 | 1031 | 1040 | 1142 | 1024 | 1059 | 48.11 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For AZU1 (Source: Uniprot.Org, NCBI)
Gene Name
AZU1
Full Name
Azurocidin
Weight
26.886kDa
Superfamily
peptidase S1 family
Alternative Names
AZAMP; AZU; AZU1; azurocidin 1; Azurocidin; CAP37; CAP37Heparin-binding protein; cationic antimicrobial protein 37; HBP; HBPCationic antimicrobial protein CAP37; HUMAZUR; NAZC; neutrophil azurocidin AZU1 AZAMP, AZU, CAP37, HBP, HUMAZUR, NAZC, hHBP azurocidin 1 azurocidin|cationic antimicrobial protein 37|cationic antimicrobial protein CAP37|heparin-binding protein|neutrophil azurocidin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on AZU1, check out the AZU1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for AZU1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Azurocidin ELISA Kit PicoKine® (EK1161)
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No publications found for EK1161
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Human Azurocidin ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated Azurocidin antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Azurocidin antigen?
B. Walker
Verified customer
Asked: 2020-12-14
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-12-14
Question
Q: Can Azurocidin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
J. Kumar
Verified customer
Asked: 2020-11-30
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-11-30
Question
Q: can I use citrate plasma as samples in Human Azurocidin Picokine® ELISA Kit (Catalog # EK1161)?
G. Nelson
Verified customer
Asked: 2020-01-02
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Azurocidin causing disruption of its protein structure. Azurocidin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Azurocidin ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-01-02
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
H. Lewis
Verified customer
Asked: 2019-01-25
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-01-25
Question
Q: which procedure should I follow in order to thaw whole blood sample for Azurocidin ELISA after freezing?
W. Bhatt
Verified customer
Asked: 2018-10-15
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Azurocidin for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-10-15
Question
Q: can you recommend the dilution ratio of serum samples for detection of Azurocidin in Human serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
T. Carter
Verified customer
Asked: 2018-02-19
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Azurocidin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-02-19
Question
Dear Sir/Madam, Greeting of the day! Kindly tell us Human Heparin Binding Protein/Azurocidin Elisa kit Cat No.EK1161, Also works is Urine or not, Customer wants this product in Urine, Kindly confirm it, Send its Procedure Sheet,
Verified Customer
Verified customer
Asked: 2016-11-28
Answer
Our lab technicians haven't
Boster Scientific Support
Answered: 2016-11-28
