Human EMMPRIN/CD147/BSG PicoKine® Quick ELISA Kit

Product Name

Human EMMPRIN/CD147/BSG PicoKine® Quick ELISA Kit

See all BSG products

SKU/Catalog Number

FEK0751

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human EMMPRIN/CD147/BSG PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human EMMPRIN/CD147/BSG in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. Sensitivity: 2 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human EMMPRIN/CD147/BSG PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0751)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: T25-H205

Sensitivity

<2 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0751 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0751 is reactive to BSG in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk

Application Guarantee

FEK0751 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of BSG

Basigin is a member of the immunoglobulin superfamily that is also known as EMMPRIN, short for extracellular matrix metalloproteinase inducer, and recently has been designated CD147(cluster of differentiation 147). It is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition(Miyauchi et al., 1991; Kanekura et al., 1991).This protein is a determinant for the Ok blood group system. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin(CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.

Kit Components

Catalog number	Description	Quantity	Volume
FEK0751-CAP	Anti-tag Pre-coated 96-well Strip Microplate	1	12 strips of 8 wells
EK0751-ST	Human BSG Standard	2	10 ng/tube
FEK0751-A	Human BSG Antibody A	1	3ml
FEK0751-B	Human BSG Antibody B	1	3ml
AR1106-1	Sample Diluent	1	15ml
AR1106-7	TBS-T Wash Buffer (25x)	1	12ml
AR1104	Color Developing Reagent (TMB)	1	10ml
AR1105	Stop Solution	1	10ml
PLA-SEA	Adhesive Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional)
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.
• Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1,000	2,000
O.D.	0.029	0.097	0.155	0.262	0.484	0.879	1.548	2.261

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of BSG in ELISA kits from four different production batches/lots.

Gene/Protein Information For BSG (Source: Uniprot.Org, NCBI)

Alternative Names

5F7; basigin (Ok blood group); Basigin; BSG; CD147 antigen; CD147; Collagenase stimulatory factor; EMMPRIN; EMMPRINTCSF; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; OK blood group antigen; OK; Tumor cell-derived collagenase stimulatory factor BSG 5F7, CD147, EMMPRIN, EMPRIN, HAb18G, OK, SLC7A11, TCSF basigin (Ok blood group) basigin|OK blood group antigen|collagenase stimulatory factor|extracellular matrix metalloproteinase inducer|hepatoma-associated antigen|leukocyte activation antigen M6|tumor cell-derived collagenase stimulatory factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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