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Product Info Summary
| SKU: | EK2099 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human M-Cadherin-15 CDH15 ELISA Kit PicoKine®
View all M-Cadherin/Cadherin-15 ELISA kits
SKU/Catalog Number
EK2099
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human M-Cadherin-15 CDH15 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CDH15 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human M-Cadherin-15 CDH15 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2099)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: L61-A606
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2099 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2099 is reactive to CDH15 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2099 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of M-Cadherin/Cadherin-15
Cadherin-15 is a protein that in humans is encoded by the CDH15 gene. It is mapped to 16q24.3. This gene is a member of the cadherin superfamily of genes, encoding calcium-dependent intercellular adhesion glycoproteins. Cadherins consist of an extracellular domain containing 5 cadherin domains, a transmembrane region, and a conserved cytoplasmic domain. Transcripts from this particular cadherin are expressed in myoblasts and upregulated in myotubule-forming cells. The protein is thought to be essential for the control of morphogenetic processes, specifically myogenesis, and may provide a trigger for terminal muscle cell differentiation.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2099-CAP | Anti-Human CDH15 Pre-coated 96-well strip microplate | 1 |
| EK2099-ST | Human CDH15 Standard | 2 vials, 10 ng/tube |
| EK2099-DA | Human CDH15 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.006 | 0.074 | 0.170 | 0.311 | 0.638 | 1.084 | 1.772 | 2.293 |
Data Example Images
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Human Cadherin-15 PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human M-Cadherin-15 CDH15 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 255 | 1011 | 3863 | 264 | 1095 | 4861 |
| Standard deviation | 10.46 | 43.47 | 177.70 | 13.46 | 64.61 | 325.69 |
| CV (%) | 4.1% | 4.3% | 4.6% | 5.1% | 5.9% | 6.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of M-Cadherin/Cadherin-15 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 255 | 267 | 282 | 292 | 274 | 14.12 | 5.1% |
| Sample 2 | 1011 | 892 | 904 | 880 | 921 | 52.22 | 5.6% |
| Sample 3 | 3863 | 4003 | 3436 | 3659 | 3740 | 214.05 | 5.7% |
Protein Target Info & Infographic
Gene/Protein Information For CDH15 (Source: Uniprot.Org, NCBI)
Gene Name
CDH15
Full Name
Cadherin-15
Weight
88.916kDa
Alternative Names
cadherin 15, M-cadherin (myotubule); cadherin 15, type 1, M-cadherin (myotubule); Cadherin-14; Cadherin-15; cadherin-3; CDH14; CDH14cadherin-14; CDH15; CDH3cadherin-15; CDHM; MCAD; MCadherin; M-Cadherin; MRD3; Muscle cadherin; muscle-cadherin CDH15 CDH14, CDH3, CDHM, MCAD, MRD3 cadherin 15 cadherin-15|cadherin 15, type 1, M-cadherin (myotubule)|cadherin-14|cadherin-3|muscle-cadherin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CDH15, check out the CDH15 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CDH15: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human M-Cadherin-15 CDH15 ELISA Kit PicoKine® (EK2099)
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No publications found for EK2099
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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5 Customer Q&As for Human M-Cadherin-15 CDH15 ELISA Kit PicoKine®
Question
Q: is it okay to use heparin plasma as samples in Human M-Cadherin Picokine® ELISA Kit (Catalog # EK2099)?
V. Maharaj
Verified customer
Asked: 2020-10-29
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of M-Cadherin causing degradation of its protein structure. M-Cadherin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the M-Cadherin ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-10-29
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
B. Mangal
Verified customer
Asked: 2020-04-07
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-04-07
Question
Q: if the enzyme conjugated M-Cadherin antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the M-Cadherin antigen?
Verified Customer
Verified customer
Asked: 2019-05-06
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-05-06
Question
Q: how to analyze ELISA data? I have obtained M-Cadherin level in serum.
Verified Customer
Verified customer
Asked: 2019-02-14
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-02-14
Question
Q: Can M-Cadherin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-06-25
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-06-25
