Human CD131 ELISA Kit PicoKine®

Product Name

Human CD131 ELISA Kit PicoKine®

View all Common beta Chain ELISA kits

SKU/Catalog Number

EK2171

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CD131 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CSF2RB in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CD131 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2171)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: W17-W443

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Standard Dilution Instructions

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2171 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2171 is reactive to CSF2RB in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2171 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Common beta Chain

Cytokine receptor common subunit beta is a protein that in humans is encoded by the CSF2RB gene. It is mapped to 15 E1; 15 37.36 cM. The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP).

Kit Components

Catalog Number	Description	Quantity
EK2171-CAP	Anti-Human CSF2RB Pre-coated 96-well strip microplate	1
EK2171-ST	Human CSF2RB Standard	2 vials, 10 ng/tube
EK2171-DA	Human CSF2RB Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1,000
O.D.	0.041	0.073	0.146	0.292	0.609	1.132	1.958	2.305

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CD131 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Common beta Chain in ELISA kits from four different production batches/lots.

Gene/Protein Information For CSF2RB (Source: Uniprot.Org, NCBI)

Alternative Names

beta, low-affinity; CD131 antigen; CD131; CDw131; colony stimulating factor 2 receptor, beta, low-affinity(granulocyte-macrophage); Common beta Chain; CSF2RB; cytokine receptor common subunit beta; GM-CSF R beta; GM-CSF/IL-3/IL-5 receptor common beta subunit; GM-CSF/IL-3/IL-5 receptor common beta-chain; IL-3 R beta; IL3RB; IL-5 R beta; interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3receptor, beta (high affinity) CSF2RB CD131, CDw131, IL3RB, IL5RB, SMDP5, betaGMR colony stimulating factor 2 receptor subunit beta cytokine receptor common subunit beta|GM-CSF/IL-3/IL-5 receptor common beta subunit|GM-CSF/IL-3/IL-5 receptor common beta-chain|beta common cytokine receptor|beta-GM-CSF receptor|colony stimulating factor 2 receptor beta common subunit|colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)|colony-stimulating factor-2 receptor, beta, low-affinity|interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3 receptor, beta (high affinity)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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