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Product Info Summary
| SKU: | EK2168 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human CHRDL2 ELISA Kit PicoKine®
View all Chordin-like 2/CHRDL2 ELISA kits
SKU/Catalog Number
EK2168
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CHRDL2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CHRDL2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CHRDL2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2168)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: L21-T429
Sensitivity
<10 pg/ml
Assay Range
93.7 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2168 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2168 is reactive to CHRDL2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2168 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Chordin-like 2/CHRDL2
This gene encodes a member of the chordin family of proteins. Chordin family members are secreted proteins that share a cysteine-rich pro-collagen repeat domain and associate with members of the transforming growth factor beta superfamily. In vitro assays demonstrate a direct interaction between the encoded protein and human activin A. This gene is expressed in many tissues including osteoblasts, where it is differentially expressed during differentiation. In addition, its expression is upregulated in human osteoarthritic joint cartilage, suggesting a role in adult cartilage regeneration.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2168-CAP | Anti-Human CHRDL2 Pre-coated 96-well strip microplate | 1 |
| EK2168-ST | Human CHRDL2 Standard | 2 vials, 10 ng/tube |
| EK2168-DA | Human CHRDL2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1,500 | 3,000 | 6,000 |
| O.D. | 0.057 | 0.120 | 0.258 | 0.531 | 0.767 | 1.082 | 1.619 | 2.199 |
Data Example Images
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Human CHRDL2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CHRDL2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 102 | 332 | 844 | 118 | 432 | 941 |
| Standard deviation | 5.41 | 14.94 | 43.04 | 5.07 | 22.03 | 44.23 |
| CV(%) | 5.3% | 4.5% | 5.1% | 4.3% | 5.1% | 4.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Chordin-like 2/CHRDL2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 102 | 122 | 131 | 142 | 124 | 5.47 | 4.4% |
| Sample 2 | 332 | 365 | 377 | 296 | 343 | 17.47 | 5.1% |
| Sample 3 | 844 | 912 | 1010 | 954 | 930 | 49.29 | 5.3% |
Protein Target Info & Infographic
Gene/Protein Information For CHRDL2 (Source: Uniprot.Org, NCBI)
Gene Name
CHRDL2
Full Name
Chordin-like protein 2
Weight
47.495kDa
Alternative Names
BNF-1; Chordin like 2; Chordin-like 2; chordin-like protein 2; CHRDL2 CHRDL2 BNF1, CHL2 chordin like 2 chordin-like protein 2|breast tumor novel factor 1|chordin-related protein 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CHRDL2, check out the CHRDL2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CHRDL2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CHRDL2 ELISA Kit PicoKine® (EK2168)
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