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Product Info Summary
| SKU: | FEK0696 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human CD26/DPP4 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0696
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD26/DPP4 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CD26/DPP4 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine. Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD26/DPP4 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0696)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: D34-P766
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0696 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0696 is reactive to DPP4 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine
Application Guarantee
FEK0696 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of DPP4
Dipeptidyl peptidase-4(DPP4), also known as adenosine deaminase complexing protein 2 or CD26(cluster of differentiation 26) is a protein that, in humans, is encoded by the DPP4 gene. By fluorescence in situ hybridization, the CD26 gene was mapped to 2q24.3. DPP4 plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. Furthermore, it appears to work as a suppressor in the development of cancer and tumours. CD26/ DPP4 plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0696-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0696-ST | Human DPP4 Standard | 2 | 20 ng/tube |
| FEK0696-A | Human DPP4 Antibody A | 1 | 3ml |
| FEK0696-B | Human DPP4 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 | 20,000 |
| O.D. | 0.017 | 0.123 | 0.217 | 0.396 | 0.769 | 1.356 | 1.966 | 2.396 |
Data Example Images
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Human DPP4 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 482 | 2761 | 8848 | 497 | 2879 | 8816 |
| Standard deviation | 32.78 | 124.25 | 575.12 | 29.32 | 155.47 | 696.46 |
| CV(%) | 6.8% | 4.5% | 6.5% | 5.9% | 5.4% | 7.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of DPP4 in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 482 | 550 | 492 | 487 | 503 | 30.67 | 6.1% |
| Sample 2 | 2761 | 2826 | 2924 | 2841 | 2838 | 76.63 | 2.7% |
| Sample 3 | 8848 | 9902 | 8546 | 9412 | 9177 | 504.74 | 5.5% |
Protein Target Info & Infographic
Gene/Protein Information For DPP4 (Source: Uniprot.Org, NCBI)
Gene Name
DPP4
Full Name
Dipeptidyl peptidase 4
Weight
88.279kDa
Superfamily
peptidase S9B family
Alternative Names
ADABP; ADCP-2; ADCP2DPP IV; Adenosine deaminase complexing protein 2TP103; CD26 antigen; CD26; CD26T-cell activation antigen CD26; dipeptidyl peptidase 4; Dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidyl-peptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP4; DPPIV; EC 3.4.14.5 DPP4 ADABP, ADCP2, CD26, DPPIV, TP103 dipeptidyl peptidase 4 dipeptidyl peptidase 4|ADCP-2|DPP IV|Gly-Pro naphthylamidase|Post-proline dipeptidyl aminopeptidase IV|T-cell activation antigen CD26|Xaa-Pro-dipeptidylaminopeptidase|adenosine deaminase complexing protein 2|dipeptidyl peptidase IV|dipeptidylpeptidase 4|dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on DPP4, check out the DPP4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for DPP4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD26/DPP4 PicoKine® Quick ELISA Kit (FEK0696)
Hello CJ!
FEK0696 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effects of isoliensinine on angiotensin II-induced proliferation of porcine coronary arterial smooth muscle cells
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