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Product Info Summary
| SKU: | M00597 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CD26/DPP4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00597
BM5083 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD26/DPP4 Rabbit Monoclonal Antibody catalog # M00597. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD26/DPP4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00597)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABOC-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD26
Reactive Species
M00597 is reactive to DPP4 in Human, Mouse, Rat
Observed Molecular Weight
110 kDa
Calculated molecular weight
88.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00597 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB:rat lung tissue
Validation Images & Assay Conditions
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Western blot analysis of CD26 using anti-CD26 antibody (M00597).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD26 antigen affinity purified monoclonal antibody (M00597) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD26 at approximately 110 kDa. The expected band size for CD26 is at 88 kDa.
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SXD remarkably affected the ferroptosis-related markers in CoCl 2 -induced hypoxic H9c2 cells. (A–D) mRNA expressions of ACSL4, GPX4, DPP4, and HMOX1 in different groups, separately. (E) Protein expressions of ACSL4, GPX4, DPP4, and HMOX1 in different groups. *P < 0.05, **P < 0.01, and ***P < 0.001.
Index in PubMed under a CC BY license. PMID: 40365322
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Molecular docking results. (A) Structure of quercetin. (B, C) Molecular docking of quercetin with DPP4 (B) and HMOX1 (C) . (D) Results of the CETSA assay.
Index in PubMed under a CC BY license. PMID: 40365322
Specific Publications For Anti-CD26/DPP4 Rabbit Monoclonal Antibody (M00597)
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Customer Q&As
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3 Customer Q&As for Anti-CD26/DPP4 Rabbit Monoclonal Antibody
Question
We are currently using anti-CD26 Rabbit Monoclonal antibody M00597 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
The anti-CD26 Rabbit Monoclonal antibody (M00597) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-12
Question
We have observed staining in human prostate uterus. Do you have any suggestions? Is anti-CD26 Rabbit Monoclonal antibody supposed to stain prostate uterus positively?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
From what I have seen in literature prostate uterus does express DPP4. From what I have seen in Uniprot.org, DPP4 is expressed in tendon of biceps brachii, liver, colon, peripheral blood, placenta, prostate uterus, kidney, plasma, among other tissues. Regarding which tissues have DPP4 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 1347043, 1977364
Kidney, Pubmed ID: 8096237
Liver, Pubmed ID: 1352704, 19159218, 24275569
Peripheral blood, Pubmed ID: 1352530
Placenta, Pubmed ID: 7927537
Plasma, Pubmed ID: 16335952
Prostate, and Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-16
Question
Our team were well pleased with the WB result of your anti-CD26 Rabbit Monoclonal antibody. However we have been able to see positive staining in plasma dipeptidyl peptidase 4 soluble form: using this antibody. Is that expected? Could you tell me where is DPP4 supposed to be expressed?
K. Kulkarni
Verified customer
Asked: 2018-03-08
Answer
Based on literature, plasma does express DPP4. Generally DPP4 expresses in dipeptidyl peptidase 4 soluble form:, cell membrane. Regarding which tissues have DPP4 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 1347043, 1977364
Kidney, Pubmed ID: 8096237
Liver, Pubmed ID: 1352704, 19159218, 24275569
Peripheral blood, Pubmed ID: 1352530
Placenta, Pubmed ID: 7927537
Plasma, Pubmed ID: 16335952
Prostate, and Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-03-08
