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Product Info Summary
| SKU: | EK0754 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants |
Product info
Product Name
Human eNOS/NOS3 ELISA Kit PicoKine®
SKU/Catalog Number
EK0754
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human eNOS/NOS3 Kit PicoKine™ (96 Tests). Quantitate Human NOS3 in cell culture supernatants. Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human eNOS/NOS3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0754)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: G2-P1203
Sensitivity
<10 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0754 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0754 is reactive to NOS3 in Human samples
Validated Sample Types
cell culture supernatants
Application Guarantee
EK0754 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of eNOS
Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is an enzyme that in humans is encoded by the NOS3 gene located in the 7q35-7q36 region of chromosome 7. Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0754-CAP | Anti-Human NOS3 Pre-coated 96-well strip microplate | 1 |
| EK0754-ST | Human NOS3 Standard | 2 vials, 10 ng/tube |
| EK0754-DA | Human NOS3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 78 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 |
| O.D. | 0.022 | 0.089 | 0.161 | 0.278 | 0.492 | 1.121 | 1.431 | 2.091 |
Data Example Images
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Human NOS3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect eNOS, Dilution ratio of 1:1, concentration in cell culture supernatants is less than the lowest standard, 78 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human eNOS/NOS3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 112 | 365 | 1362 | 125 | 344 | 1399 |
| Standard deviation | 3.47 | 10.22 | 43.58 | 4.13 | 15.48 | 53.16 |
| CV(%) | 3.1% | 2.8% | 3.2% | 3.3% | 4.5% | 3.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of eNOS in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 112 | 127 | 118 | 123 | 120 | 4.68 | 3.9% |
| Sample 2 | 365 | 359 | 381 | 377 | 371 | 19.27 | 5.2% |
| Sample 3 | 1362 | 1369 | 1489 | 1546 | 1442 | 31.71 | 2.2% |
Protein Target Info & Infographic
Gene/Protein Information For NOS3 (Source: Uniprot.Org, NCBI)
Gene Name
NOS3
Full Name
Nitric oxide synthase, endothelial
Weight
133.275kDa
Superfamily
NOS family
Alternative Names
Constitutive NOS; EC 1.14.13.39; ECNOS; Endothelial NOS; endothelial; eNOS; eNOScNOS; nitric oxide synthase 3 (endothelial cell); NOS type III; NOS3; NOSIII NOS3 ECNOS, eNOS nitric oxide synthase 3 nitric oxide synthase, endothelial|EC-NOS|NOS type III|NOSIII|cNOS|constitutive NOS|endothelial NOS|nitric oxide synthase 3 (endothelial cell)|nitric oxide synthase 3 transcript variant eNOS-delta20|nitric oxide synthase 3 transcript variant eNOS-delta20-21|nitric oxide synthase 3 transcript variant eNOS-delta21
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on NOS3, check out the NOS3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NOS3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human eNOS/NOS3 ELISA Kit PicoKine® (EK0754)
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